Fluorescence lifetime and depth estimation of a tumor site for functional imaging purposes

Osnat Harbater, Moshe Ben-David, Israel Gannot

Research output: Contribution to journalArticlepeer-review

Abstract

Cancerous cells have irregular environmental conditions, such as temperature and pH, which distinguish them from their surroundings. Fluorescence lifetime imaging using near-IR (NIR) fluorescent probes, whose lifetime value is sensitive to pH and temperature, enables the estimation of these values, and provides functional information about the tumor. The lifetime value, extracted from the time-resolved intensity decay curve, combines the photon time delays, caused by the photon time of flight, and the intrinsic lifetime in which we are interested. In this study, we present a model, based on the diffusion approximation of the radiation transport equation, for extracting both the depth of an NIR fluorescent probe, and its intrinsic lifetime value, from a fluorescence time decay curve. The model was validated for different inclusion depths, fluorescent lifetime values, and scattering coefficients using a time-resolved Monte Carlo simulation. Our reported results are the first step toward performing functional imaging using fluorescence lifetime in vivo measurements.

Original languageEnglish (US)
Article number5437301
Pages (from-to)981-988
Number of pages8
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume16
Issue number4
DOIs
StatePublished - Jul 2010
Externally publishedYes

Keywords

  • Diffusion theory
  • fluorescence
  • optical imaging
  • optical scattering
  • time-domain measurements

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Fluorescence lifetime and depth estimation of a tumor site for functional imaging purposes'. Together they form a unique fingerprint.

Cite this