TY - JOUR
T1 - Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant
AU - Hawkins, Anita L.
AU - Jones, Richard J.
AU - Zehnbauer, Barbara A.
AU - Zicha, Milada S.
AU - Collector, Michael J.
AU - Sharkis, Saul J.
AU - Griffin, Constance A.
N1 - Funding Information:
This work was partially funded by NIH Grants No. 1R01-HL46533-01 (to R.J.J. and S.J.S.) and No. 5POI-CA15396-17 (to A.L.H. and C.A.G.).
PY - 1992/12
Y1 - 1992/12
N2 - The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.
AB - The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.
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U2 - 10.1016/0165-4608(92)90345-9
DO - 10.1016/0165-4608(92)90345-9
M3 - Article
C2 - 1486564
AN - SCOPUS:0027057660
SN - 0165-4608
VL - 64
SP - 145
EP - 148
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -