Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

Anita L. Hawkins, Richard J. Jones, Barbara A. Zehnbauer, Milada S. Zicha, Michael J. Collector, Saul J. Sharkis, Constance A. Griffin

Research output: Contribution to journalArticlepeer-review

Abstract

The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalCancer Genetics and Cytogenetics
Volume64
Issue number2
DOIs
StatePublished - Dec 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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