Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification

Christoph Lengauer, Eric D. Green, Thomas Cremer

Research output: Contribution to journalArticle

Abstract

Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size. The resulting DNA probes were used in chromosome in situ suppression (CISS) hybridization experiments. Strong fluorescent signals on both chromatids indicated the localization of specific YAC clones, while two clearly distinguishable signals were observed in ≥90% of diploid nuclei Signal intensities were generally comparable to those observed using chromosome-specific alphoid DNA probes. This approach will facilitate the rapid mapping of YAC clones and their use in chromosome analysis at all stages of the cell cycle.

Original languageEnglish (US)
Pages (from-to)826-828
Number of pages3
JournalGenomics
Volume13
Issue number3
DOIs
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification'. Together they form a unique fingerprint.

  • Cite this