Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification

Christoph Lengauer, Eric D. Green, Thomas Cremer

Research output: Contribution to journalArticle

Abstract

Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size. The resulting DNA probes were used in chromosome in situ suppression (CISS) hybridization experiments. Strong fluorescent signals on both chromatids indicated the localization of specific YAC clones, while two clearly distinguishable signals were observed in ≥90% of diploid nuclei Signal intensities were generally comparable to those observed using chromosome-specific alphoid DNA probes. This approach will facilitate the rapid mapping of YAC clones and their use in chromosome analysis at all stages of the cell cycle.

Original languageEnglish (US)
Pages (from-to)826-828
Number of pages3
JournalGenomics
Volume13
Issue number3
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Yeast Artificial Chromosomes
DNA Probes
Fluorescence In Situ Hybridization
Clone Cells
Chromosomes
Polymerase Chain Reaction
Chromatids
Diploidy
In Situ Hybridization
Cell Cycle
Yeasts

ASJC Scopus subject areas

  • Genetics

Cite this

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. / Lengauer, Christoph; Green, Eric D.; Cremer, Thomas.

In: Genomics, Vol. 13, No. 3, 1992, p. 826-828.

Research output: Contribution to journalArticle

Lengauer, Christoph ; Green, Eric D. ; Cremer, Thomas. / Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. In: Genomics. 1992 ; Vol. 13, No. 3. pp. 826-828.
@article{59d997259c524393a6d5d67fcc303c9b,
title = "Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification",
abstract = "Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size. The resulting DNA probes were used in chromosome in situ suppression (CISS) hybridization experiments. Strong fluorescent signals on both chromatids indicated the localization of specific YAC clones, while two clearly distinguishable signals were observed in ≥90{\%} of diploid nuclei Signal intensities were generally comparable to those observed using chromosome-specific alphoid DNA probes. This approach will facilitate the rapid mapping of YAC clones and their use in chromosome analysis at all stages of the cell cycle.",
author = "Christoph Lengauer and Green, {Eric D.} and Thomas Cremer",
year = "1992",
doi = "10.1016/0888-7543(92)90160-T",
language = "English (US)",
volume = "13",
pages = "826--828",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification

AU - Lengauer, Christoph

AU - Green, Eric D.

AU - Cremer, Thomas

PY - 1992

Y1 - 1992

N2 - Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size. The resulting DNA probes were used in chromosome in situ suppression (CISS) hybridization experiments. Strong fluorescent signals on both chromatids indicated the localization of specific YAC clones, while two clearly distinguishable signals were observed in ≥90% of diploid nuclei Signal intensities were generally comparable to those observed using chromosome-specific alphoid DNA probes. This approach will facilitate the rapid mapping of YAC clones and their use in chromosome analysis at all stages of the cell cycle.

AB - Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size. The resulting DNA probes were used in chromosome in situ suppression (CISS) hybridization experiments. Strong fluorescent signals on both chromatids indicated the localization of specific YAC clones, while two clearly distinguishable signals were observed in ≥90% of diploid nuclei Signal intensities were generally comparable to those observed using chromosome-specific alphoid DNA probes. This approach will facilitate the rapid mapping of YAC clones and their use in chromosome analysis at all stages of the cell cycle.

UR - http://www.scopus.com/inward/record.url?scp=0026725180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026725180&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(92)90160-T

DO - 10.1016/0888-7543(92)90160-T

M3 - Article

VL - 13

SP - 826

EP - 828

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -