Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters

Mounir Bouhifd, Maurice P. Whelan, Marc Aprahamian

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel prototype instrument for mutli-spectral imaging applications is described. The device is based on an acoustooptic tuneable filter (AOTF) that can be easily attached to many standard imaging systems (e.g. endoscope or fluorescence microscope). The instrument developed offers significant advantages over typical fixed-filter based systems in terms of flexibility, performance and diagnostic potential. The selected AOTF was designed to have a large acceptance aperture suitable for imaging applications. Any filtering centre-wavelength in the visible range (450 to 700nm) can be rapidly selected by either random access or by continuous tuning thus providing a versatile performance. The prototype instrument has been demonstrated for in-vivo applications where it was attached to the eyepiece of a commercial endoscope allowing simultaneous white light and fluorescence endoscopy. Autofluorescence of endogenous protoporphyrin IX (PpIK), a biomarker of diseased tissues undergoing an inflammatory response, was mapped in vivo on a rat model. The AOTF device was also coupled to the viewing port of a commercial fluorescence microscope thus realising a powerful fluorescence imaging spectrometer capable of detecting and mapping fluorescent biomolecules in vitro.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsH.J. Byrne, E. Lewis, B.D. MacCraith, E. McGlynn
Pages185-193
Number of pages9
Volume5826
DOIs
StatePublished - 2005
Externally publishedYes
EventOpto-Ireland 2005: Optical Sensing and Spectroscopy - Dublin, Ireland
Duration: Apr 4 2005Apr 6 2005

Other

OtherOpto-Ireland 2005: Optical Sensing and Spectroscopy
CountryIreland
CityDublin
Period4/4/054/6/05

Fingerprint

acousto-optics
Acoustooptical effects
Endoscopy
Optics
Fluorescence
Spectroscopy
Imaging techniques
filters
endoscopes
fluorescence
spectroscopy
Microscopes
microscopes
prototypes
eyepieces
random access
biomarkers
imaging spectrometers
Biomolecules
Biomarkers

Keywords

  • AOTF filtering
  • Autofluorescence
  • Endoscopy
  • Fluorescence imaging
  • Microscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Bouhifd, M., Whelan, M. P., & Aprahamian, M. (2005). Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters. In H. J. Byrne, E. Lewis, B. D. MacCraith, & E. McGlynn (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5826, pp. 185-193). [23] https://doi.org/10.1117/12.605088

Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters. / Bouhifd, Mounir; Whelan, Maurice P.; Aprahamian, Marc.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / H.J. Byrne; E. Lewis; B.D. MacCraith; E. McGlynn. Vol. 5826 2005. p. 185-193 23.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bouhifd, M, Whelan, MP & Aprahamian, M 2005, Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters. in HJ Byrne, E Lewis, BD MacCraith & E McGlynn (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5826, 23, pp. 185-193, Opto-Ireland 2005: Optical Sensing and Spectroscopy, Dublin, Ireland, 4/4/05. https://doi.org/10.1117/12.605088
Bouhifd M, Whelan MP, Aprahamian M. Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters. In Byrne HJ, Lewis E, MacCraith BD, McGlynn E, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5826. 2005. p. 185-193. 23 https://doi.org/10.1117/12.605088
Bouhifd, Mounir ; Whelan, Maurice P. ; Aprahamian, Marc. / Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters. Proceedings of SPIE - The International Society for Optical Engineering. editor / H.J. Byrne ; E. Lewis ; B.D. MacCraith ; E. McGlynn. Vol. 5826 2005. pp. 185-193
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