Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model

Z. G. Wang, D. B. Durand, M. Schoenberg, Y. T. Pan

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: We describe the technique of fluorescence image guided optical coherence tomography (FG-OCT). We examined its ability to enhance specificity and sensitivity for the noninvasive diagnosis of early bladder cancer. Materials and Methods: Transitional cell carcinoma was developed in 54 Fisher 344 female rats by intravesical methyl-nitroso-urea instillations. Two or three rats were diagnosed sequentially by 5-ALA (5-aminolevulinic acid hydrochloride) induced fluorescence imaging, cross-sectional OCT and histological microscopy weekly during weeks 11 to 33 following initial methyl-nitroso-urea instillation to track the course of carcinogenesis. Results: The specificity of fluorescence detection was significantly enhanced by FG-OCT (53% and 93%, respectively, p <0.0001). The sensitivity of fluorescence detection and FG-OCT was 79% and 100%, respectively. Conclusions: FG-OCT cystoscopy has the potential to diagnose early bladder cancer with high sensitivity and specificity with drastically decreased imaging time compared to that of white light guided OCT cystoscopy.

Original languageEnglish (US)
Pages (from-to)2376-2381
Number of pages6
JournalJournal of Urology
Volume174
Issue number6
DOIs
StatePublished - Dec 2005

Keywords

  • Bladder
  • Bladder neoplasms
  • Carcinoma, transitional cell
  • Rats, inbred F344
  • Tomography, optical coherence

ASJC Scopus subject areas

  • Urology

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