Fluorescence-based resource for semiautomated genomic analyses using microsatellite markers

Roy C. Levitt, Matthew B. Kiser, Carl Dragwa, Anne E. Jedlicka, Jienfiang Xu, Deborah A. Meyers, James R. Hudson

Research output: Contribution to journalArticlepeer-review

Abstract

To facilitate the practical application of highly efficient semiautomated methods for general application in genomic analyses, we have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 cM, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 10% of the genome lies beyond 20 cM of the nearest marker. Since this genomic analysis system is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems, the 5 groups of 18 markers can be detected concurrently. This multiplex detection provides a throughput of 1944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including linkage, cancer genetics, forensics, and cytogenetics.

Original languageEnglish (US)
Pages (from-to)361-365
Number of pages5
JournalGenomics
Volume24
Issue number2
DOIs
StatePublished - Nov 15 1994

ASJC Scopus subject areas

  • Genetics

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