Fluorescence-Based Resource for Semiautomated Genomic Analyses Using Microsatellite Markers

Roy C. Levitt, Matthew B. Kiser, Carl Dragwa, Anne Elizabeth Jedlicka, Jienfiang Xu, Deborah A. Meyers, James R. Hudson

Research output: Contribution to journalArticle

Abstract

To facilitate the practical application of highly efficient semiautomated methods for general application in genomic analyses, we have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 cM, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 10% of the genome lies beyond 20 cM of the nearest marker. Since this genomic analysis system is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems, the 5 groups of 18 markers can be detected concurrently. This multiplex detection provides a throughput of 1944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including linkage, cancer genetics, forensics, and cytogenetics.

Original languageEnglish (US)
Pages (from-to)361-365
Number of pages5
JournalGenomics
Volume24
Issue number2
DOIs
StatePublished - Nov 15 1994

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Microsatellite Repeats
Forensic Genetics
Fluorescence
Genome
Genetic Linkage
Y Chromosome
X Chromosome
Cytogenetics
Color
Genotype
Research
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Levitt, R. C., Kiser, M. B., Dragwa, C., Jedlicka, A. E., Xu, J., Meyers, D. A., & Hudson, J. R. (1994). Fluorescence-Based Resource for Semiautomated Genomic Analyses Using Microsatellite Markers. Genomics, 24(2), 361-365. https://doi.org/10.1006/geno.1994.1628

Fluorescence-Based Resource for Semiautomated Genomic Analyses Using Microsatellite Markers. / Levitt, Roy C.; Kiser, Matthew B.; Dragwa, Carl; Jedlicka, Anne Elizabeth; Xu, Jienfiang; Meyers, Deborah A.; Hudson, James R.

In: Genomics, Vol. 24, No. 2, 15.11.1994, p. 361-365.

Research output: Contribution to journalArticle

Levitt, RC, Kiser, MB, Dragwa, C, Jedlicka, AE, Xu, J, Meyers, DA & Hudson, JR 1994, 'Fluorescence-Based Resource for Semiautomated Genomic Analyses Using Microsatellite Markers', Genomics, vol. 24, no. 2, pp. 361-365. https://doi.org/10.1006/geno.1994.1628
Levitt, Roy C. ; Kiser, Matthew B. ; Dragwa, Carl ; Jedlicka, Anne Elizabeth ; Xu, Jienfiang ; Meyers, Deborah A. ; Hudson, James R. / Fluorescence-Based Resource for Semiautomated Genomic Analyses Using Microsatellite Markers. In: Genomics. 1994 ; Vol. 24, No. 2. pp. 361-365.
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