Fluid shear modulates the molecular interactions between polymorphonuclear leukocytes and colon carcinomas

Research output: Contribution to journalConference articlepeer-review

Abstract

This study compares the kinetics, adhesion efficiency, and molecular requirements of polymorphonuclear leukocyte (PMN) binding to two colon adenocarcinoma cell-lines, the ICAM-1-negative/sLex-bearing LS174T and the ICAM-1-expressing/sLex-low HCT-8 as a function of hydrodynamic shear rate (50-1600 sec-1). The efficiency of both PMN homotypic and PMN-colon carcinoma heterotypic interactions, defined as the fraction of collisions between respective cells that result in aggregation, decreases sharply at low shear. At higher shear, PMN homotypic efficiency remains relatively unchanged, while heterotypic efficiencies continue to decrease with PMN binding to LS174T cells being more efficient than to HCT-8 cells. The relative contributions of LFA-1 and Mac-1 as well as their respective ligands on HCT-8 cells to heterotypic aggregation were investigated in the presence of fluid shear.

Original languageEnglish (US)
Pages (from-to)734-735
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Keywords

  • HCT-8 cells
  • ICAM-1
  • Leukocytes
  • LFA-1
  • LS174T cells
  • Mac-1
  • Shear

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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