Fluid shear- and time-dependent modulation of molecular interactions between PMNs and colon carcinomas

Research output: Contribution to journalArticlepeer-review

Abstract

This study compares the effects of fluid shear on the kinetics, adhesion efficiency, stability, and molecular requirements of polymorphonuclear leukocyte (PMN) binding to two colon adenocarcinoma cell-lines, the CD54-negative/sLex-bearing LS174T cells and the CD54-expressing/sLex-low HCT-8 cells. The efficiency of PMN-colon carcinoma heteroaggregation decreases with increasing shear, with PMNs binding HCT-8 more efficiently than LS174T cells at low shear (50-200 s-1). In the low shear regime, CD11b is sufficient to mediate PMN binding to LS174T cells. In contrast, both CD11a and CD11b contribute to PMN-HCT-8 heteroaggregation, with CD54 on HCT-8 cells acting as a CD11a ligand at early time points. At high shear, only PMN-LS174T heteroaggregation occurs, which is initiated by PMN L-selectin binding to a sialylated, O-linked, protease-sensitive ligand on LS174T cells. PMN-LS174T heteroaggregation is primarily dependent on the intercellular contact duration (or shear rate), whereas PMN-HCT-8 binding is a function of both the intercellular contact duration and the applied force (or shear stress). Cumulatively, these studies suggest that fluid shear modulates the kinetics and molecular mechanisms of PMN-colon carcinoma cell aggregation.

Original languageEnglish (US)
Pages (from-to)C1133-C1143
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number4 52-4
DOIs
StatePublished - Oct 2002

Keywords

  • CD11a/CD18
  • CD11b/CD18
  • HCT-8 cells
  • L-selectin
  • LS174T cells
  • Polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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