Fluctuations in phosphoribosyl pyrophosphate levels in monolayer tumor cell lines. Effects of drugs

G. J. Peters, E. Laurensse, A. Leyva, H. M. Pinedo

Research output: Contribution to journalArticle

Abstract

The concentration of PRPP (phosphoribosyl pyrophosphate) measured in tumor cells grown in monolayer showed a large variation with the various harvesting methods examined, including trypsinization. This variation could be reduced by a 1-h incubation of trypsinized cells as a suspension in Dulbecco's medium. After this preincubation these cell suspensions were suitable for the study of modulation of PRPP. One μM methotrexate caused a 2-3-fold increase and 1 mM N-phosphonoacetyl-L-aspartate a slight increase, but inosine and deoxyinosine drastically reduced PRPP concentrations. 5-Fluorouracil had no effect. This study demonstrates that metabolic parameters such as PRPP concentrations can be studied conveniently in suspensions of cells which are commonly cultivated as monolayers.

Original languageEnglish (US)
Pages (from-to)277-280
Number of pages4
JournalFEBS Letters
Volume170
Issue number2
DOIs
StatePublished - May 21 1984
Externally publishedYes

Keywords

  • Modulation
  • Monolayer tumor cell
  • Phosphoribosyl pyrophosphate
  • Purine
  • Pyrimidine antimetabolite

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Fluctuations in phosphoribosyl pyrophosphate levels in monolayer tumor cell lines. Effects of drugs'. Together they form a unique fingerprint.

  • Cite this