Flow-induced responses in cat isolated pulmonary arteries

Larissa A. Shimoda, Nan A. Norins, Jane A. Madden

Research output: Contribution to journalArticlepeer-review

Abstract

Isolated, cannulated, endothelium-intact cat pulmonary arteries, averaging 692 ± 104 μm in diameter, were set at a transmural pressure of 10 mmHg and monitored with a video system. Intraluminal flow was increased in steps from 0 to 1.6 ml/min by using a syringe pump. An electronic system held pressure constant by changing outflow resistance. Flow-diameter curves were generated in physiological saline solution. At constant transmural pressure, the arteries constricted in response to increased intraluminal flow. Constriction was not affected by removing extracellular Ca2+ but was abolished after treatment with ryanodine to deplete intracellular Ca2+ stores, with the endothelin-1 synthesis inhibitor phosphoramidon, with the endothelin A-receptor antagonist BQ-123, with the protein kinase C inhibitor staurosporine, or with glutaralde-hyde to reduce endothelial cell deformability. The results indicate that isolated pulmonary arteries can constrict in response to intraluminal flow and suggest that constriction is mediated by endothelin-1 and depends on intracellular Ca2+ release and protein kinase C activation.

Original languageEnglish (US)
Pages (from-to)1617-1622
Number of pages6
JournalJournal of applied physiology
Volume83
Issue number5
StatePublished - Nov 1 1997
Externally publishedYes

Keywords

  • BQ-123
  • Endothelin-1
  • Shear stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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