Flow-dependent cytosolic acidification of vascular endothelial cells

Roy Ziegelstein, Linda Cheng, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

Abstract

Hemodynamic shear stress affects endothelial cell structure and function, but little is know about the signal transduction mechanisms involved in these processes. The effect laminar shear stress on cytosolic pH (pHi) was examined in rat aortic endothelial cells cultured in glass capillary tubes. Shear stress forces led to a rapid decrease in pHi (maximal effect 0.09 pH unit at 13.4 dynes per square centimeter). Removal of specific ions addition of exchange inhibitors suggests that in vascular endothelial cells shear stress forces activate both an alkali extruder, sodium ion-independent chloride-bicarbonate ion exchange, and an acid extruder, sodium-hydrogen ion exchange; the net effect in physiologic buffer with the bicarbonate ion is a decrease in pHi.

Original languageEnglish (US)
Pages (from-to)656-659
Number of pages4
JournalScience
Volume258
Issue number5082
StatePublished - Oct 23 1992
Externally publishedYes

Fingerprint

Ion Exchange
Endothelial Cells
Bicarbonates
Sodium
Alkalies
Glass
Protons
Chlorides
Signal Transduction
Buffers
Hemodynamics
Ions
Acids

ASJC Scopus subject areas

  • General

Cite this

Ziegelstein, R., Cheng, L., & Capogrossi, M. C. (1992). Flow-dependent cytosolic acidification of vascular endothelial cells. Science, 258(5082), 656-659.

Flow-dependent cytosolic acidification of vascular endothelial cells. / Ziegelstein, Roy; Cheng, Linda; Capogrossi, Maurizio C.

In: Science, Vol. 258, No. 5082, 23.10.1992, p. 656-659.

Research output: Contribution to journalArticle

Ziegelstein, R, Cheng, L & Capogrossi, MC 1992, 'Flow-dependent cytosolic acidification of vascular endothelial cells', Science, vol. 258, no. 5082, pp. 656-659.
Ziegelstein R, Cheng L, Capogrossi MC. Flow-dependent cytosolic acidification of vascular endothelial cells. Science. 1992 Oct 23;258(5082):656-659.
Ziegelstein, Roy ; Cheng, Linda ; Capogrossi, Maurizio C. / Flow-dependent cytosolic acidification of vascular endothelial cells. In: Science. 1992 ; Vol. 258, No. 5082. pp. 656-659.
@article{e7ae866a90c54aa5ad148a0e19d32fc8,
title = "Flow-dependent cytosolic acidification of vascular endothelial cells",
abstract = "Hemodynamic shear stress affects endothelial cell structure and function, but little is know about the signal transduction mechanisms involved in these processes. The effect laminar shear stress on cytosolic pH (pHi) was examined in rat aortic endothelial cells cultured in glass capillary tubes. Shear stress forces led to a rapid decrease in pHi (maximal effect 0.09 pH unit at 13.4 dynes per square centimeter). Removal of specific ions addition of exchange inhibitors suggests that in vascular endothelial cells shear stress forces activate both an alkali extruder, sodium ion-independent chloride-bicarbonate ion exchange, and an acid extruder, sodium-hydrogen ion exchange; the net effect in physiologic buffer with the bicarbonate ion is a decrease in pHi.",
author = "Roy Ziegelstein and Linda Cheng and Capogrossi, {Maurizio C.}",
year = "1992",
month = "10",
day = "23",
language = "English (US)",
volume = "258",
pages = "656--659",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5082",

}

TY - JOUR

T1 - Flow-dependent cytosolic acidification of vascular endothelial cells

AU - Ziegelstein, Roy

AU - Cheng, Linda

AU - Capogrossi, Maurizio C.

PY - 1992/10/23

Y1 - 1992/10/23

N2 - Hemodynamic shear stress affects endothelial cell structure and function, but little is know about the signal transduction mechanisms involved in these processes. The effect laminar shear stress on cytosolic pH (pHi) was examined in rat aortic endothelial cells cultured in glass capillary tubes. Shear stress forces led to a rapid decrease in pHi (maximal effect 0.09 pH unit at 13.4 dynes per square centimeter). Removal of specific ions addition of exchange inhibitors suggests that in vascular endothelial cells shear stress forces activate both an alkali extruder, sodium ion-independent chloride-bicarbonate ion exchange, and an acid extruder, sodium-hydrogen ion exchange; the net effect in physiologic buffer with the bicarbonate ion is a decrease in pHi.

AB - Hemodynamic shear stress affects endothelial cell structure and function, but little is know about the signal transduction mechanisms involved in these processes. The effect laminar shear stress on cytosolic pH (pHi) was examined in rat aortic endothelial cells cultured in glass capillary tubes. Shear stress forces led to a rapid decrease in pHi (maximal effect 0.09 pH unit at 13.4 dynes per square centimeter). Removal of specific ions addition of exchange inhibitors suggests that in vascular endothelial cells shear stress forces activate both an alkali extruder, sodium ion-independent chloride-bicarbonate ion exchange, and an acid extruder, sodium-hydrogen ion exchange; the net effect in physiologic buffer with the bicarbonate ion is a decrease in pHi.

UR - http://www.scopus.com/inward/record.url?scp=0026478845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026478845&partnerID=8YFLogxK

M3 - Article

C2 - 1329207

AN - SCOPUS:0026478845

VL - 258

SP - 656

EP - 659

JO - Science

JF - Science

SN - 0036-8075

IS - 5082

ER -