Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple ig heavy-chain lineages

James R R Whittle, Adam K. Wheatley, Lan Wu, Daniel Lingwood, Masaru Kanekiyo, Steven S. Ma, Sandeep R. Narpala, Hadi M. Yassine, Gregory M. Frank, Jonathan W. Yewdell, Julie E. Ledgerwood, Chih Jen Wei, Adrian B. McDermott, Barney S. Graham, Richard A. Koup, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

An understanding of the antigen-specific B-cell response to the influenza virus hemagglutinin (HA) is critical to the development of universal influenza vaccines, but it has not been possible to examine these cells directly because HA binds to sialic acid (SA) on most cell types. Here, we use structure-based modification of HA to isolate HA-specific B cells by flow cytometry and characterize the features of HA stem antibodies (Abs) required for their development. Incorporation of a previously described mutation (Y98F) to the receptor binding site (RBS) causes HA to bind only those B cells that express HA-specific Abs, but it does not bind nonspecifically to B cells, and this mutation has no effect on the binding of broadly neutralizing Abs to the RBS. To test the specificity of the Y98F mutation, we first demonstrated that previously described HA nanoparticles mediate hemagglutination and then determined that the Y98F mutation eliminates this activity. Cloning of immunoglobulin genes from HA-specific B cells isolated from a single human subject demonstrates that vaccination with H5N1 influenza virus can elicit B cells expressing stem monoclonal Abs (MAbs). Although these MAbs originated mostly from the IGHV1-69 germ line, a reasonable proportion derived from other genes. Analysis of stem Abs provides insight into the maturation pathways of IGVH1-69-derived stem Abs. Furthermore, this analysis shows that multiple non-IGHV1-69 stem Abs with a similar neutralizing breadth develop after vaccination in humans, suggesting that the HA stem response can be elicited in individuals with non-stem-reactive IGHV1-69 alleles.

Original languageEnglish (US)
Pages (from-to)4047-4057
Number of pages11
JournalJournal of Virology
Volume88
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

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Immunoglobulin Heavy Chains
Hemagglutinins
hemagglutinins
flow cytometry
Flow Cytometry
Vaccination
vaccination
antibodies
stems
Antibodies
B-lymphocytes
B-Lymphocytes
mutation
Mutation
Orthomyxoviridae
binding sites
Binding Sites
immunoglobulin genes
H5N1 Subtype Influenza A Virus
Immunoglobulin Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Whittle, J. R. R., Wheatley, A. K., Wu, L., Lingwood, D., Kanekiyo, M., Ma, S. S., ... Nabel, G. J. (2014). Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple ig heavy-chain lineages. Journal of Virology, 88(8), 4047-4057. https://doi.org/10.1128/JVI.03422-13

Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple ig heavy-chain lineages. / Whittle, James R R; Wheatley, Adam K.; Wu, Lan; Lingwood, Daniel; Kanekiyo, Masaru; Ma, Steven S.; Narpala, Sandeep R.; Yassine, Hadi M.; Frank, Gregory M.; Yewdell, Jonathan W.; Ledgerwood, Julie E.; Wei, Chih Jen; McDermott, Adrian B.; Graham, Barney S.; Koup, Richard A.; Nabel, Gary J.

In: Journal of Virology, Vol. 88, No. 8, 2014, p. 4047-4057.

Research output: Contribution to journalArticle

Whittle, JRR, Wheatley, AK, Wu, L, Lingwood, D, Kanekiyo, M, Ma, SS, Narpala, SR, Yassine, HM, Frank, GM, Yewdell, JW, Ledgerwood, JE, Wei, CJ, McDermott, AB, Graham, BS, Koup, RA & Nabel, GJ 2014, 'Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple ig heavy-chain lineages', Journal of Virology, vol. 88, no. 8, pp. 4047-4057. https://doi.org/10.1128/JVI.03422-13
Whittle, James R R ; Wheatley, Adam K. ; Wu, Lan ; Lingwood, Daniel ; Kanekiyo, Masaru ; Ma, Steven S. ; Narpala, Sandeep R. ; Yassine, Hadi M. ; Frank, Gregory M. ; Yewdell, Jonathan W. ; Ledgerwood, Julie E. ; Wei, Chih Jen ; McDermott, Adrian B. ; Graham, Barney S. ; Koup, Richard A. ; Nabel, Gary J. / Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple ig heavy-chain lineages. In: Journal of Virology. 2014 ; Vol. 88, No. 8. pp. 4047-4057.
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