Abstract
Background Cutaneous B-cell lymphoproliferative lesions can pose diagnostic challenges. This study investigates the utlility of flow cytometry in 42 cases of suspected cutaneous B-cell lymphoma. Methods All available cases were reviewed [World Health Organization-European Organization for Research and Treatment of Cancer (WHO-EORTC) classification]. Flow cytometry, immunohistochemistry and polymerase chain reaction-immunoglobulin H (PCR-IgH) analysis of blood and/or lesional skin were performed on primary cutaneous B-cell lymphoma (pcBCL, 17 cases), secondary cutaneous BCL (scBCL, 8 cases) and atypical lymphoid hyperplasia (ALH, 17 cases). Results Flow cytometry of skin detected a B-cell clone in 3/13 cases of ALH, 8/8 cases of pcBCL and 4/4 cases of scBCL, while PCR detected a clone in 3/14 cases of ALH, 4/15 cases of pcBCL and 6/8 cases of scBCL. Of eight cases of pcBCL analyzed by both methods, all eight were positive by flow while only three were positive by PCR. All cases positive by PCR were also positive by flow. Of five cases with both flow and light chain immunohistochemistry, all five showed light chain restriction by flow, while only two were positive by immunohistochemistry. Conclusion Flow cytometry is more sensitive than PCR in detecting B-cell lymphoproliferative disorders (12/12 cases, 100% vs. 10/23 cases, 43%; p <0.001). Furthermore, flow cytometry complements immunohistochemistry in the detection of light chain restriction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 918-928 |
| Number of pages | 11 |
| Journal | Journal of Cutaneous Pathology |
| Volume | 39 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2012 |
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Keywords
- cutaneous B-cell lymphoma
- flow cytometry
- gene rearrangement
ASJC Scopus subject areas
- Dermatology
- Pathology and Forensic Medicine
- Histology
Cite this
Flow cytometry of lesional skin enhances the evaluation of cutaneous B-cell lymphomas. / Wu, Julie Michelle; Vonderheid, Eric; Gocke, Christopher; Moresi, Jean Margaret; Liegeois, Nanette; Borowitz, Michael J.
In: Journal of Cutaneous Pathology, Vol. 39, No. 10, 10.2012, p. 918-928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow cytometry of lesional skin enhances the evaluation of cutaneous B-cell lymphomas
AU - Wu, Julie Michelle
AU - Vonderheid, Eric
AU - Gocke, Christopher
AU - Moresi, Jean Margaret
AU - Liegeois, Nanette
AU - Borowitz, Michael J
PY - 2012/10
Y1 - 2012/10
N2 - Background Cutaneous B-cell lymphoproliferative lesions can pose diagnostic challenges. This study investigates the utlility of flow cytometry in 42 cases of suspected cutaneous B-cell lymphoma. Methods All available cases were reviewed [World Health Organization-European Organization for Research and Treatment of Cancer (WHO-EORTC) classification]. Flow cytometry, immunohistochemistry and polymerase chain reaction-immunoglobulin H (PCR-IgH) analysis of blood and/or lesional skin were performed on primary cutaneous B-cell lymphoma (pcBCL, 17 cases), secondary cutaneous BCL (scBCL, 8 cases) and atypical lymphoid hyperplasia (ALH, 17 cases). Results Flow cytometry of skin detected a B-cell clone in 3/13 cases of ALH, 8/8 cases of pcBCL and 4/4 cases of scBCL, while PCR detected a clone in 3/14 cases of ALH, 4/15 cases of pcBCL and 6/8 cases of scBCL. Of eight cases of pcBCL analyzed by both methods, all eight were positive by flow while only three were positive by PCR. All cases positive by PCR were also positive by flow. Of five cases with both flow and light chain immunohistochemistry, all five showed light chain restriction by flow, while only two were positive by immunohistochemistry. Conclusion Flow cytometry is more sensitive than PCR in detecting B-cell lymphoproliferative disorders (12/12 cases, 100% vs. 10/23 cases, 43%; p <0.001). Furthermore, flow cytometry complements immunohistochemistry in the detection of light chain restriction.
AB - Background Cutaneous B-cell lymphoproliferative lesions can pose diagnostic challenges. This study investigates the utlility of flow cytometry in 42 cases of suspected cutaneous B-cell lymphoma. Methods All available cases were reviewed [World Health Organization-European Organization for Research and Treatment of Cancer (WHO-EORTC) classification]. Flow cytometry, immunohistochemistry and polymerase chain reaction-immunoglobulin H (PCR-IgH) analysis of blood and/or lesional skin were performed on primary cutaneous B-cell lymphoma (pcBCL, 17 cases), secondary cutaneous BCL (scBCL, 8 cases) and atypical lymphoid hyperplasia (ALH, 17 cases). Results Flow cytometry of skin detected a B-cell clone in 3/13 cases of ALH, 8/8 cases of pcBCL and 4/4 cases of scBCL, while PCR detected a clone in 3/14 cases of ALH, 4/15 cases of pcBCL and 6/8 cases of scBCL. Of eight cases of pcBCL analyzed by both methods, all eight were positive by flow while only three were positive by PCR. All cases positive by PCR were also positive by flow. Of five cases with both flow and light chain immunohistochemistry, all five showed light chain restriction by flow, while only two were positive by immunohistochemistry. Conclusion Flow cytometry is more sensitive than PCR in detecting B-cell lymphoproliferative disorders (12/12 cases, 100% vs. 10/23 cases, 43%; p <0.001). Furthermore, flow cytometry complements immunohistochemistry in the detection of light chain restriction.
KW - cutaneous B-cell lymphoma
KW - flow cytometry
KW - gene rearrangement
UR - http://www.scopus.com/inward/record.url?scp=84866060525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866060525&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0560.2012.01975.x
DO - 10.1111/j.1600-0560.2012.01975.x
M3 - Article
C2 - 22845743
AN - SCOPUS:84866060525
VL - 39
SP - 918
EP - 928
JO - Journal of Cutaneous Pathology
JF - Journal of Cutaneous Pathology
SN - 0303-6987
IS - 10
ER -