Flow cytometric DNA analysis of primary and concurrent metastatic squamous cell carcinoma of the head and neck

Julian A. Kim, Valerie L. Moore, Mukund S. Didolkar, Jose V. Ordonez, Robert A. Van Wesep, Charles M. Suter

Research output: Contribution to journalArticle

Abstract

Adequate flow cytometric DNA analysis comparing primary and concurrent metastatic squamous cell carcinoma of the head and neck has not been done in the past. The purpose of this study was to define any differences between the primary and concurrent metastasis of each patient with respect to flow cytometric parameters and histologic grade. Paraffin-embedded archival specimens from 28 patients with primary and metastatic tumors were prepared into nuclei and analyzed by flow cytometry using human lymphocyte standards. The mean DNA index was 0.82 for primary tumors and 0.83 for the metastases. Aneuploidy was found in 68 percent of primary tumors and in 82 percent of metastases. The percentage of cells in the proliferative fraction was 40.4 in the primary tumors and 24.5 in the metastases. A direct correlation was found between the differentiation of the primary and metastatic tumors. No survival difference was discovered among the flow cytometric parameters and histologic grade. We conclude that there is no difference between the primary and concurrent metastasis in squamous cell carcinoma of the head and neck with regard to DNA index, aneuploidy, or histologic grade.

Original languageEnglish (US)
Pages (from-to)288-291
Number of pages4
JournalAmerican Journal of Surgery
Volume158
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

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Neoplasm Metastasis
DNA
Aneuploidy
Neoplasms
Paraffin
Flow Cytometry
Carcinoma, squamous cell of head and neck
Lymphocytes
Survival

ASJC Scopus subject areas

  • Surgery

Cite this

Kim, J. A., Moore, V. L., Didolkar, M. S., Ordonez, J. V., Van Wesep, R. A., & Suter, C. M. (1989). Flow cytometric DNA analysis of primary and concurrent metastatic squamous cell carcinoma of the head and neck. American Journal of Surgery, 158(4), 288-291. https://doi.org/10.1016/0002-9610(89)90117-7

Flow cytometric DNA analysis of primary and concurrent metastatic squamous cell carcinoma of the head and neck. / Kim, Julian A.; Moore, Valerie L.; Didolkar, Mukund S.; Ordonez, Jose V.; Van Wesep, Robert A.; Suter, Charles M.

In: American Journal of Surgery, Vol. 158, No. 4, 1989, p. 288-291.

Research output: Contribution to journalArticle

Kim, Julian A. ; Moore, Valerie L. ; Didolkar, Mukund S. ; Ordonez, Jose V. ; Van Wesep, Robert A. ; Suter, Charles M. / Flow cytometric DNA analysis of primary and concurrent metastatic squamous cell carcinoma of the head and neck. In: American Journal of Surgery. 1989 ; Vol. 158, No. 4. pp. 288-291.
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AU - Van Wesep, Robert A.

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