Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Francesco Boin, Maria Letizia Giardino Torchia, Ivan Borrello, Kimberly A. Noonan, Matthew Neil, Mark J. Soloski, Raffaello Cimbro

Research output: Contribution to journalArticlepeer-review

Abstract

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

Original languageEnglish (US)
Article numbere0188916
JournalPloS one
Volume12
Issue number11
DOIs
StatePublished - Nov 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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