@article{22b9199c2a834bc1bcd5d1566ccfc369,
title = "Flow cytometric discrimination of seven lineage markers by using two fluorochromes",
abstract = "Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.",
author = "Francesco Boin and {Giardino Torchia}, {Maria Letizia} and Ivan Borrello and Noonan, {Kimberly A.} and Matthew Neil and Soloski, {Mark J.} and Raffaello Cimbro",
note = "Funding Information: This study was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases, https://www.niams.nih.gov/, award number P30-AR053503; The Stabler Foundation, www.stablerfoundation.org; National Institute of Allergy and Infectious Disease, www.niaid.nih.gov, T32AI007247; Nina Ireland Program for Lung Health, (NIPLH), https://pulmonary.ucsf.edu/ ireland/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2017 Boin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2017",
month = nov,
doi = "10.1371/journal.pone.0188916",
language = "English (US)",
volume = "12",
journal = "PloS one",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",
}