Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Francesco Boin, Maria Letizia Giardino Torchia, Ivan M Borrello, Kimberly Noonan, Matthew Neil, Mark J Soloski, Raffaello Cimbro

Research output: Contribution to journalArticle

Abstract

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

Original languageEnglish (US)
Article numbere0188916
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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