Flow cytometric discrimination of seven lineage markers by using two fluorochromes

Francesco Boin, Maria Letizia Giardino Torchia, Ivan M Borrello, Kimberly Noonan, Matthew Neil, Mark J Soloski, Raffaello Cimbro

Research output: Contribution to journalArticle

Abstract

Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

Original languageEnglish (US)
Article numbere0188916
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

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T-cells
fluorescent dyes
Fluorescent Dyes
T-lymphocytes
Cells
Immunologic Techniques
T-Lymphocytes
Immunophenotyping
Flow cytometry
natural killer cells
immunologic techniques
Natural Killer Cells
monocytes
Population
B-lymphocytes
flow cytometry
Monocytes
Flow Cytometry
B-Lymphocytes
Cell Count

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Flow cytometric discrimination of seven lineage markers by using two fluorochromes. / Boin, Francesco; Giardino Torchia, Maria Letizia; Borrello, Ivan M; Noonan, Kimberly; Neil, Matthew; Soloski, Mark J; Cimbro, Raffaello.

In: PLoS One, Vol. 12, No. 11, e0188916, 01.11.2017.

Research output: Contribution to journalArticle

Boin, Francesco ; Giardino Torchia, Maria Letizia ; Borrello, Ivan M ; Noonan, Kimberly ; Neil, Matthew ; Soloski, Mark J ; Cimbro, Raffaello. / Flow cytometric discrimination of seven lineage markers by using two fluorochromes. In: PLoS One. 2017 ; Vol. 12, No. 11.
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