Flexible micro spring array device for high-throughput enrichment of viable circulating tumor cells

Ramdane A. Harouaka, Ming Da Zhou, Yin Ting Yeh, Waleed J. Khan, Avisnata Das, Xin Liu, Christine C. Christ, David T. Dicker, Tara S. Baney, Jussuf T. Kaifi, Chandra P. Belani, Cristina I. Truica, Wafik S. El-Deiry, Jeffrey P. Allerton, Si Yang Zheng

Research output: Contribution to journalArticle

Abstract

Background: The dissemination of circulating tumor cells (CTCs) that cause metastases in distant organs accounts for the majority of cancer-related deaths. CTCs have been established as a cancer biomarker of known prognostic value. The enrichment of viable CTCs for ex vivo analysis could further improve cancer diagnosis and guide treatment selection. We designed a new flexible micro spring array (FMSA) device for the enrichment of viable CTCs independent of antigen expression. methods: Unlike previous microfiltration devices, flexible structures at the micro scale minimize cell damage to preserve viability, while maximizing throughput to allow rapid enrichment directly from whole blood with no need for sample preprocessing. Device performance with respect to capture efficiency, enrichment against leukocytes, viability, and proliferability was characterized. CTCs and CTC microclusters were enriched from clinical samples obtained from breast, lung, and colorectal cancer patients. results: The FMSA device enriched tumor cells with 90% capture efficiency, higher than 104 enrichment, and better than 80% viability from 7.5-mL whole blood samples in

Original languageEnglish (US)
Pages (from-to)323-333
Number of pages11
JournalClinical Chemistry
Volume60
Issue number2
DOIs
StatePublished - Feb 2014
Externally publishedYes

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Circulating Neoplastic Cells
Tumors
Cells
Throughput
Equipment and Supplies
Blood
Neoplasms
Tumor Biomarkers
Microfiltration
Flexible structures
Colorectal Neoplasms
Lung Neoplasms
Leukocytes
Breast Neoplasms
Neoplasm Metastasis
Antigens

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Medicine(all)

Cite this

Harouaka, R. A., Zhou, M. D., Yeh, Y. T., Khan, W. J., Das, A., Liu, X., ... Zheng, S. Y. (2014). Flexible micro spring array device for high-throughput enrichment of viable circulating tumor cells. Clinical Chemistry, 60(2), 323-333. https://doi.org/10.1373/clinchem.2013.206805

Flexible micro spring array device for high-throughput enrichment of viable circulating tumor cells. / Harouaka, Ramdane A.; Zhou, Ming Da; Yeh, Yin Ting; Khan, Waleed J.; Das, Avisnata; Liu, Xin; Christ, Christine C.; Dicker, David T.; Baney, Tara S.; Kaifi, Jussuf T.; Belani, Chandra P.; Truica, Cristina I.; El-Deiry, Wafik S.; Allerton, Jeffrey P.; Zheng, Si Yang.

In: Clinical Chemistry, Vol. 60, No. 2, 02.2014, p. 323-333.

Research output: Contribution to journalArticle

Harouaka, RA, Zhou, MD, Yeh, YT, Khan, WJ, Das, A, Liu, X, Christ, CC, Dicker, DT, Baney, TS, Kaifi, JT, Belani, CP, Truica, CI, El-Deiry, WS, Allerton, JP & Zheng, SY 2014, 'Flexible micro spring array device for high-throughput enrichment of viable circulating tumor cells', Clinical Chemistry, vol. 60, no. 2, pp. 323-333. https://doi.org/10.1373/clinchem.2013.206805
Harouaka, Ramdane A. ; Zhou, Ming Da ; Yeh, Yin Ting ; Khan, Waleed J. ; Das, Avisnata ; Liu, Xin ; Christ, Christine C. ; Dicker, David T. ; Baney, Tara S. ; Kaifi, Jussuf T. ; Belani, Chandra P. ; Truica, Cristina I. ; El-Deiry, Wafik S. ; Allerton, Jeffrey P. ; Zheng, Si Yang. / Flexible micro spring array device for high-throughput enrichment of viable circulating tumor cells. In: Clinical Chemistry. 2014 ; Vol. 60, No. 2. pp. 323-333.
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AU - Christ, Christine C.

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AU - Belani, Chandra P.

AU - Truica, Cristina I.

AU - El-Deiry, Wafik S.

AU - Allerton, Jeffrey P.

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