Flagellar elongation and gene expression in Chlamydomonas reinhardtii

Goran Periz, Darshita Dharia, Steven H. Miller, Laura R. Keller

Research output: Contribution to journalArticle

Abstract

Lithium (Li+) affects the physiology of cells from a broad range of organisms including plants and both vertebrate and invertebrate animals. Although its effects result presumably from changes in gene expression elicited by its interaction with intracellular signal transduction pathways, the molecular mechanisms of Li+ action are not well understood. The biflagellate green alga Chlamydomonas reinhardtii is an ideal genetic model for the integration of the effects on Li+ on signal transduction, gene expression, and aspects of flagellar biogenesis. Li+ causes C. reinhardtii flagella to elongate to ∼1.4 times their normal length and blocks flagellar motility (S. Nakamura, H. Tabino, and M. K. Kojima, Cell Struct. Funct. 12:369-374, 1987). We report here that Li+ treatment increases the abundance of several flagellar mRNAs, including α- and β-tubulin and pcf3-21. Li+-induced flagellar gene expression occurs in cells pretreated with cycloheximide, suggesting that the abundance change is a response that does not require new protein synthesis. Deletion analysis of the flagellar α1-tubulin gene promoter showed that sequences necessary for Li+-induced expression differed from those for acid shock induction and contain a consensus binding site for CREB/ATF and AP-1 transcription factors. These studies suggest potential promoter elements, candidate factors, and signal transduction pathways that may coordinate the C. reinhardtii cellular response to Li+.

Original languageEnglish (US)
Pages (from-to)1411-1420
Number of pages10
JournalEukaryotic Cell
Volume6
Issue number8
DOIs
StatePublished - Aug 2007

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Chlamydomonas reinhardtii
signal transduction
Signal Transduction
tubulin
Gene Expression
gene expression
Tubulin Modulators
promoter regions
cell physiology
Cell Physiological Phenomena
Chlorophyta
Flagella
lithium
Genetic Models
Transcription Factor AP-1
cycloheximide
Invertebrates
Cycloheximide
Tubulin
flagellum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Flagellar elongation and gene expression in Chlamydomonas reinhardtii. / Periz, Goran; Dharia, Darshita; Miller, Steven H.; Keller, Laura R.

In: Eukaryotic Cell, Vol. 6, No. 8, 08.2007, p. 1411-1420.

Research output: Contribution to journalArticle

Periz, Goran ; Dharia, Darshita ; Miller, Steven H. ; Keller, Laura R. / Flagellar elongation and gene expression in Chlamydomonas reinhardtii. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 8. pp. 1411-1420.
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