First field trial of an immunoradiometric assay for the detection of malaria sporozoites in mosquitoes

F. H. Collins, F. Zavala, P. M. Graves, A. H. Cochrane, R. W. Gwadz, J. Akoh, R. S. Nussenzweig

Research output: Contribution to journalArticlepeer-review

Abstract

An immunoradiometric assay (IRMA) using a monoclonal antibody to the major surface protein of Plasmodium falciparum sporozoites was used to assess the P. falciparum sporozoite rate in a West African population of Anopheles gambiae (s.l.). Unlike current dissection techniques, the IRMA could detect sporozoite antigen in dried as well as fresh mosquitoes. In a controlled comparison, the sensitivity of the IRMA was comparable that of the dissection technique. Additionally, the IRMA was species specific and quantitative. Sensitivity of the assay was sufficient to detect sporozoite infections resulting from the development of a single oocyst.

Original languageEnglish (US)
Pages (from-to)538-543
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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