First draft genome sequence of the pathogenic fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Ruibang Luo, Aleksey Zimin, Rachael Workman, Yunfan Fan, Geo Pertea, Nina Grossman, Maggie P. Wear, Bei Jia, Heather Miller, Arturo Casadevall, Winston Timp, Sean X. Zhang, Steven L. Salzberg

Research output: Contribution to journalArticlepeer-review

Abstract

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,630,066 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8,656 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

Original languageEnglish (US)
JournalUnknown Journal
DOIs
StatePublished - Aug 3 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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