Abstract
Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsedfield gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.
Original language | English (US) |
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Pages (from-to) | 3831-3836 |
Number of pages | 6 |
Journal | G3: Genes, Genomes, Genetics |
Volume | 7 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 2017 |
Keywords
- Fungal genomics
- Genome assembly
- Nanopore sequencing
- Pathogen genomics
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)