First draft genome sequence of the pathogenic fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Ruibang Luo, Aleksey Zimin, Rachael Workman, Yunfan Fan, Geo Pertea, Nina Grossman, Maggie P. Wear, Bei Jia, Heather Miller, Arturo Casadevall, Winston Timp, Sean Xiang Zhang, Steven L Salzberg

Research output: Contribution to journalArticle

Abstract

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsedfield gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

Original languageEnglish (US)
Pages (from-to)3831-3836
Number of pages6
JournalG3: Genes, Genomes, Genetics
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2017

    Fingerprint

Keywords

  • Fungal genomics
  • Genome assembly
  • Nanopore sequencing
  • Pathogen genomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this