Firefly luciferase as a tool in molecular and cell biology

Stephen J. Gould; Suresh Subramani

doi:10.1016/0003-2697(88)90353-3

Firefly luciferase as a tool in molecular and cell biology

Stephen J. Gould, Suresh Subramani

Research output: Contribution to journal › Review article › peer-review

267 Scopus citations

Abstract

The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.

Original language	English (US)
Pages (from-to)	5-13
Number of pages	9
Journal	Analytical biochemistry
Volume	175
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(88)90353-3
State	Published - Nov 15 1988
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Firefly luciferase as a tool in molecular and cell biology",

abstract = "The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.",

author = "Gould, {Stephen J.} and Suresh Subramani",

note = "Funding Information: This work was supported by Grants GM31253 and CA01062 and a March of Dimes grant (No. 108 1) to S.S. S.J.G. was supported by a fellowship from the Powell Foundation.",

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AU - Subramani, Suresh

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N2 - The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.

AB - The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.

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Firefly luciferase as a tool in molecular and cell biology

Abstract

ASJC Scopus subject areas

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