Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates

Martin L. Basch, Rogers M. Brown, Hsin I. Jen, Fatih Semerci, Frederic Depreux, Renée K. Edlund, Hongyuan Zhang, Christine R. Norton, Thomas Gridley, Susan E. Cole, Angelika Doetzlhofer, Mirjana Maletic-Savatic, Neil Segil, Andrew K. Groves

Research output: Contribution to journalArticle

Abstract

The signals that induce the organ of Corti and define its boundaries in the cochlea are poorly understood. We show that two Notch modifiers, Lfng and Mfng, are transiently expressed precisely at the neural boundary of the organ of Corti. Cre-Lox fate mapping shows this region gives rise to inner hair cells and their associated inner phalangeal cells. Mutation of Lfng and Mfng disrupts this boundary, producing unexpected duplications of inner hair cells and inner phalangeal cells. This phenotype is mimicked by other mouse mutants or pharmacological treatments that lower but not abolish Notch signaling. However, strong disruption of Notch signaling causes a very different result, generating many ectopic hair cells at the expense of inner phalangeal cells. Our results show that Notch signaling is finely calibrated in the cochlea to produce precisely tuned levels of signaling that first set the boundary of the organ of Corti and later regulate hair cell development.

Original languageEnglish (US)
Article numbere19921
JournaleLife
Volume5
Issue numberDECEMBER2016
DOIs
StatePublished - Dec 14 2016

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Organ of Corti
Inner Auditory Hair Cells
Tuning
Cells
Cochlea
Pharmacology
Phenotype
Mutation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Basch, M. L., Brown, R. M., Jen, H. I., Semerci, F., Depreux, F., Edlund, R. K., ... Groves, A. K. (2016). Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates. eLife, 5(DECEMBER2016), [e19921]. https://doi.org/10.7554/eLife.19921

Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates. / Basch, Martin L.; Brown, Rogers M.; Jen, Hsin I.; Semerci, Fatih; Depreux, Frederic; Edlund, Renée K.; Zhang, Hongyuan; Norton, Christine R.; Gridley, Thomas; Cole, Susan E.; Doetzlhofer, Angelika; Maletic-Savatic, Mirjana; Segil, Neil; Groves, Andrew K.

In: eLife, Vol. 5, No. DECEMBER2016, e19921, 14.12.2016.

Research output: Contribution to journalArticle

Basch, ML, Brown, RM, Jen, HI, Semerci, F, Depreux, F, Edlund, RK, Zhang, H, Norton, CR, Gridley, T, Cole, SE, Doetzlhofer, A, Maletic-Savatic, M, Segil, N & Groves, AK 2016, 'Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates', eLife, vol. 5, no. DECEMBER2016, e19921. https://doi.org/10.7554/eLife.19921
Basch ML, Brown RM, Jen HI, Semerci F, Depreux F, Edlund RK et al. Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates. eLife. 2016 Dec 14;5(DECEMBER2016). e19921. https://doi.org/10.7554/eLife.19921
Basch, Martin L. ; Brown, Rogers M. ; Jen, Hsin I. ; Semerci, Fatih ; Depreux, Frederic ; Edlund, Renée K. ; Zhang, Hongyuan ; Norton, Christine R. ; Gridley, Thomas ; Cole, Susan E. ; Doetzlhofer, Angelika ; Maletic-Savatic, Mirjana ; Segil, Neil ; Groves, Andrew K. / Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates. In: eLife. 2016 ; Vol. 5, No. DECEMBER2016.
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