Fimbria-fornix transections selectively down-regulate subtypes of glutamate transporter and glutamate receptor proteins in septum and hippocampus

Stephen D. Ginsberg, Jeffrey D. Rothstein, Donald L. Price, Lee J. Martin

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of CNS axotomy on glutamate transporter and glutamate receptor expression were evaluated in adult rats following unilateral fimbria-fornix transections. The septum and hippocampus were collected at 3, 7, 14, and 30 days postlesion. Homogenates were immunoblotted by using antibodies directed against glutamate transporters (GLT-1, GLAST, and EAAC1) and glutamate receptors (GluR1, GluR2/3, GluR6/7, and NMDAR1), and they were assayed for glutamate transport by D-[3H]aspartate binding. GLT-1 was decreased at 7 and 14 days postlesion within the ipsilateral septum and at 7 days postlesion in the hippocampus. GLAST was decreased within the ipsilateral septum and hippocampus at 7 and 14 days postlesion. No postlesion alterations in EAAC1 immunoreactivity were observed. D[3H]Aspartate binding was decreased at 7, 14, and 30 days postlesion within the ipsilateral septum and 14 days postlesion in the hippocampus. GluR2/3 expression was down- regulated at 30 days postlesion within the ipsilateral septum, whereas GluR1, GluR6/7, and NMDAR1 immunoreactivity was unchanged. In addition, no alterations in glutamate receptor expression were detected within hippocampal homogenates. This study demonstrates a selective down-regulation of primarily glial, and not neuronal, glutamate transporters and a delayed, subtype- specific down-regulation of septal GluR2/3 receptor expression after regional deafferentation within the CNS.

Original languageEnglish (US)
Pages (from-to)1208-1216
Number of pages9
JournalJournal of Neurochemistry
Volume67
Issue number3
DOIs
StatePublished - Sep 1996

Keywords

  • Axotomy
  • Fimbria - fornix
  • Glutamate receptor
  • Glutamate transporter
  • Hippocampus
  • Septum

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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