Fight against background noise in stimulated emission depletion nanoscopy

Ye Ma; Taekjip Ha

doi:10.1088/1478-3975/ab255c

Fight against background noise in stimulated emission depletion nanoscopy

Ye Ma, Taekjip Ha

School of Medicine

Research output: Contribution to journal › Review article › peer-review

9 Scopus citations

Abstract

STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti- Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.

Original language	English (US)
Article number	051002
Journal	Physical biology
Volume	16
Issue number	5
DOIs	https://doi.org/10.1088/1478-3975/ab255c
State	Published - Sep 2019

Keywords

Background noise
Optical microscopy
STED
Superresolution

ASJC Scopus subject areas

Biophysics
Structural Biology
Molecular Biology
Cell Biology

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10.1088/1478-3975/ab255c

Cite this

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title = "Fight against background noise in stimulated emission depletion nanoscopy",

abstract = "STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti- Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.",

keywords = "Background noise, Optical microscopy, STED, Superresolution",

author = "Ye Ma and Taekjip Ha",

note = "Funding Information: We gratefully acknowledge to Kyu Young Han for a careful reading of this manuscript, Myung Hyun Jo for BJ cell sample preparation, and Jong Chan Lee for helpful discussions. This work was supported by US NSF grant PHY 1430124, NIH grant GM 112659 and GM122569 to TH. TH is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2019 IOP Publishing Ltd.",

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language = "English (US)",

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T1 - Fight against background noise in stimulated emission depletion nanoscopy

AU - Ma, Ye

AU - Ha, Taekjip

N1 - Funding Information: We gratefully acknowledge to Kyu Young Han for a careful reading of this manuscript, Myung Hyun Jo for BJ cell sample preparation, and Jong Chan Lee for helpful discussions. This work was supported by US NSF grant PHY 1430124, NIH grant GM 112659 and GM122569 to TH. TH is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: © 2019 IOP Publishing Ltd.

PY - 2019/9

Y1 - 2019/9

N2 - STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti- Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.

AB - STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti- Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.

KW - Background noise

KW - Optical microscopy

KW - STED

KW - Superresolution

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VL - 16

JO - Physical biology

JF - Physical biology

IS - 5

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Fight against background noise in stimulated emission depletion nanoscopy

Abstract

Keywords

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