Field evaluation of a coproantigen enzyme-linked immunosorbent assay for diagnosis of canine echinococcosis in a rural Andean village in Peru

Luis Lopera; Pedro L. Moro; Amanda Chavez; Glicerio Montes; Armando Gonzales; Robert H. Gilman

doi:10.1016/j.vetpar.2003.07.019

Field evaluation of a coproantigen enzyme-linked immunosorbent assay for diagnosis of canine echinococcosis in a rural Andean village in Peru

Luis Lopera, Pedro L. Moro, Amanda Chavez, Glicerio Montes, Armando Gonzales, Robert H. Gilman

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

One hundred and six dogs (61 males and 45 females) were examined for Echinococcus granulosus infection in a farming cooperative in the central highlands of Peru during November 1998. Canine echinococcosis was diagnosed using direct microscopic examinations of purged feces following arecoline purging and a coproantigen-detection enzyme-linked immunosorbent assay (ELISA) for E. granulosus. Mean age was 2 years with a range of 3 months to 9 years. The overall prevalence of canine echinococcosis using the ELISA test was 79% (84/106). Seventy-four dogs were successfully purged with arecoline. The frequency of canine echinococcosis was 82 (61/74) and 34% (25/74) by the coproantigen ELISA test and arecoline purging, respectively. The sensitivity and specificity of the coproantigen ELISA test was 88 and 95%, respectively. We found this assay to be especially advantageous in remote geographical areas. In future control programs against echinococcosis in Peru and other areas where E. granulosus is endemic the coproantigen ELISA should be used for the surveillance of the dog population.

Original language	English (US)
Pages (from-to)	37-42
Number of pages	6
Journal	Veterinary Parasitology
Volume	117
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetpar.2003.07.019
State	Published - Nov 3 2003

Keywords

Coproantigen ELISA
Diagnosis
Dog
Echinococcus granulosus
Peru

ASJC Scopus subject areas

Parasitology
General Veterinary

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10.1016/j.vetpar.2003.07.019

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title = "Field evaluation of a coproantigen enzyme-linked immunosorbent assay for diagnosis of canine echinococcosis in a rural Andean village in Peru",

abstract = "One hundred and six dogs (61 males and 45 females) were examined for Echinococcus granulosus infection in a farming cooperative in the central highlands of Peru during November 1998. Canine echinococcosis was diagnosed using direct microscopic examinations of purged feces following arecoline purging and a coproantigen-detection enzyme-linked immunosorbent assay (ELISA) for E. granulosus. Mean age was 2 years with a range of 3 months to 9 years. The overall prevalence of canine echinococcosis using the ELISA test was 79% (84/106). Seventy-four dogs were successfully purged with arecoline. The frequency of canine echinococcosis was 82 (61/74) and 34% (25/74) by the coproantigen ELISA test and arecoline purging, respectively. The sensitivity and specificity of the coproantigen ELISA test was 88 and 95%, respectively. We found this assay to be especially advantageous in remote geographical areas. In future control programs against echinococcosis in Peru and other areas where E. granulosus is endemic the coproantigen ELISA should be used for the surveillance of the dog population.",

keywords = "Coproantigen ELISA, Diagnosis, Dog, Echinococcus granulosus, Peru",

author = "Luis Lopera and Moro, {Pedro L.} and Amanda Chavez and Glicerio Montes and Armando Gonzales and Gilman, {Robert H.}",

note = "Funding Information: This study was funded by Consejo Nacional de Ciencia y Tecnologı́a (CONCYTEC). We wish to thank Dr. Peter Schantz for manuscript review and the people and authorities of the farming cooperative of Tupac Amaru in Junin, Peru.",

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AU - Moro, Pedro L.

AU - Chavez, Amanda

AU - Montes, Glicerio

AU - Gonzales, Armando

AU - Gilman, Robert H.

N1 - Funding Information: This study was funded by Consejo Nacional de Ciencia y Tecnologı́a (CONCYTEC). We wish to thank Dr. Peter Schantz for manuscript review and the people and authorities of the farming cooperative of Tupac Amaru in Junin, Peru.

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N2 - One hundred and six dogs (61 males and 45 females) were examined for Echinococcus granulosus infection in a farming cooperative in the central highlands of Peru during November 1998. Canine echinococcosis was diagnosed using direct microscopic examinations of purged feces following arecoline purging and a coproantigen-detection enzyme-linked immunosorbent assay (ELISA) for E. granulosus. Mean age was 2 years with a range of 3 months to 9 years. The overall prevalence of canine echinococcosis using the ELISA test was 79% (84/106). Seventy-four dogs were successfully purged with arecoline. The frequency of canine echinococcosis was 82 (61/74) and 34% (25/74) by the coproantigen ELISA test and arecoline purging, respectively. The sensitivity and specificity of the coproantigen ELISA test was 88 and 95%, respectively. We found this assay to be especially advantageous in remote geographical areas. In future control programs against echinococcosis in Peru and other areas where E. granulosus is endemic the coproantigen ELISA should be used for the surveillance of the dog population.

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KW - Echinococcus granulosus

KW - Peru

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Field evaluation of a coproantigen enzyme-linked immunosorbent assay for diagnosis of canine echinococcosis in a rural Andean village in Peru

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Keywords

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