Fibronectin mediates mesendodermal cell fate decisions

Paul Cheng, Peter Andersen, David Hassel, Bogac L. Kaynak, Pattraranee Limphong, Lonny Juergensen, Chulan Kwon, Deepak Srivastava

Research output: Contribution to journalArticle

Abstract

Non-cell-autonomous signals often play crucial roles in cell fate decisions during animal development. Reciprocal signaling between endoderm and mesoderm is vital for embryonic development, yet the key signals and mechanisms remain unclear. Here, we show that endodermal cells efficiently promote the emergence of mesodermal cells in the neighboring population through signals containing an essential short-range component. The endoderm-mesoderm interaction promoted precardiac mesoderm formation in mouse embryonic stem cells and involved endodermal production of fibronectin. In vivo, fibronectin deficiency resulted in a dramatic reduction of mesoderm accompanied by endodermal expansion in zebrafish embryos. This event was mediated by regulation of Wnt signaling in mesodermal cells through activation of integrin-ß1. Our findings highlight the importance of the extracellular matrix in mediating short-range signals and reveal a novel function of endoderm, involving fibronectin and its downstream signaling cascades, in promoting the emergence of mesoderm.

Original languageEnglish (US)
Pages (from-to)2587-2596
Number of pages10
JournalJournal of Cell Science
Volume126
Issue number12
DOIs
StatePublished - Jun 2013

Fingerprint

Mesoderm
Fibronectins
Endoderm
Zebrafish
Integrins
Embryonic Development
Extracellular Matrix
Embryonic Structures
Population

Keywords

  • Endoderm
  • Fibronectin
  • Integrin-β1
  • Mesoderm
  • Wnt

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cheng, P., Andersen, P., Hassel, D., Kaynak, B. L., Limphong, P., Juergensen, L., ... Srivastava, D. (2013). Fibronectin mediates mesendodermal cell fate decisions. Journal of Cell Science, 126(12), 2587-2596. https://doi.org/10.1242/jcs.136309

Fibronectin mediates mesendodermal cell fate decisions. / Cheng, Paul; Andersen, Peter; Hassel, David; Kaynak, Bogac L.; Limphong, Pattraranee; Juergensen, Lonny; Kwon, Chulan; Srivastava, Deepak.

In: Journal of Cell Science, Vol. 126, No. 12, 06.2013, p. 2587-2596.

Research output: Contribution to journalArticle

Cheng, P, Andersen, P, Hassel, D, Kaynak, BL, Limphong, P, Juergensen, L, Kwon, C & Srivastava, D 2013, 'Fibronectin mediates mesendodermal cell fate decisions', Journal of Cell Science, vol. 126, no. 12, pp. 2587-2596. https://doi.org/10.1242/jcs.136309
Cheng P, Andersen P, Hassel D, Kaynak BL, Limphong P, Juergensen L et al. Fibronectin mediates mesendodermal cell fate decisions. Journal of Cell Science. 2013 Jun;126(12):2587-2596. https://doi.org/10.1242/jcs.136309
Cheng, Paul ; Andersen, Peter ; Hassel, David ; Kaynak, Bogac L. ; Limphong, Pattraranee ; Juergensen, Lonny ; Kwon, Chulan ; Srivastava, Deepak. / Fibronectin mediates mesendodermal cell fate decisions. In: Journal of Cell Science. 2013 ; Vol. 126, No. 12. pp. 2587-2596.
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