Fibronectin fibrillogenesis regulates three-dimensional neovessel formation

Xiaoming Zhou, R. Grant Rowe, Nobuaki Hiraoka, Jerry P. George, Denis Wirtz, Deane F. Mosher, Ismo Virtanen, Michael A. Chernousov, Stephen J. Weiss

Research output: Contribution to journalArticle

Abstract

During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells establish adhesive interactions with surrounding ligands to optimally respond to soluble or matrix-bound agonists, the manner in which a randomly ordered ECM with diverse physico-mechanical properties is remodeled to support blood vessel formation has remained undefined. Herein, we demonstrate that endothelial cells initiate neovascularization by unfolding soluble fibronectin (Fn) and depositing a pericellular network of fibrils that serve to support cytoskeletal organization, actomyosin-dependent tension, and the viscoelastic properties of the embedded cells in a 3D-specific fashion. These results advance a new model wherein Fn polymerization serves as a structural scaffolding that displays adhesive ligands on a mechanically ideal substratum for promoting neovessel development.

Original languageEnglish (US)
Pages (from-to)1231-1243
Number of pages13
JournalGenes & development
Volume22
Issue number9
DOIs
StatePublished - May 1 2008

Fingerprint

Fibronectins
Endothelial Cells
Adhesives
Extracellular Matrix
Ligands
Actomyosin
Collagen Type I
Fibrin
Polymerization
Blood Vessels
Intercellular Signaling Peptides and Proteins

Keywords

  • Actomyosin
  • Angiogenesis
  • Endothelial cells
  • Extracellular matrix
  • Fibronectin

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Zhou, X., Rowe, R. G., Hiraoka, N., George, J. P., Wirtz, D., Mosher, D. F., ... Weiss, S. J. (2008). Fibronectin fibrillogenesis regulates three-dimensional neovessel formation. Genes & development, 22(9), 1231-1243. https://doi.org/10.1101/gad.1643308

Fibronectin fibrillogenesis regulates three-dimensional neovessel formation. / Zhou, Xiaoming; Rowe, R. Grant; Hiraoka, Nobuaki; George, Jerry P.; Wirtz, Denis; Mosher, Deane F.; Virtanen, Ismo; Chernousov, Michael A.; Weiss, Stephen J.

In: Genes & development, Vol. 22, No. 9, 01.05.2008, p. 1231-1243.

Research output: Contribution to journalArticle

Zhou, X, Rowe, RG, Hiraoka, N, George, JP, Wirtz, D, Mosher, DF, Virtanen, I, Chernousov, MA & Weiss, SJ 2008, 'Fibronectin fibrillogenesis regulates three-dimensional neovessel formation', Genes & development, vol. 22, no. 9, pp. 1231-1243. https://doi.org/10.1101/gad.1643308
Zhou, Xiaoming ; Rowe, R. Grant ; Hiraoka, Nobuaki ; George, Jerry P. ; Wirtz, Denis ; Mosher, Deane F. ; Virtanen, Ismo ; Chernousov, Michael A. ; Weiss, Stephen J. / Fibronectin fibrillogenesis regulates three-dimensional neovessel formation. In: Genes & development. 2008 ; Vol. 22, No. 9. pp. 1231-1243.
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