Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice

Jaepyeong Cha, Martin Paukert, Dwight E Bergles, Jin U. Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain. The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm2 cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained mice during locomotion.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8928
ISBN (Print)9780819498410
DOIs
StatePublished - 2014
EventOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics - San Francisco, CA, United States
Duration: Feb 1 2014Feb 4 2014

Other

OtherOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
CountryUnited States
CitySan Francisco, CA
Period2/1/142/4/14

Fingerprint

Functional Neuroimaging
Fluorescence microscopy
Fluorescence Microscopy
Fiber optics
brain
mice
fiber optics
Brain
Optical Imaging
microscopy
Imaging techniques
fluorescence
Fluorescence
locomotion
Locomotion
Neuroglia
Transgenic Mice
Lenses
bundles
animals

Keywords

  • Astrocyte calcium transients
  • cerebellum
  • Fiber-optic sensor
  • Fluorescence microendoscopy
  • Functional brain imaging
  • GCaMP3

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Cha, J., Paukert, M., Bergles, D. E., & Kang, J. U. (2014). Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8928). [89282N] SPIE. https://doi.org/10.1117/12.2038265

Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice. / Cha, Jaepyeong; Paukert, Martin; Bergles, Dwight E; Kang, Jin U.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8928 SPIE, 2014. 89282N.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cha, J, Paukert, M, Bergles, DE & Kang, JU 2014, Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8928, 89282N, SPIE, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, San Francisco, CA, United States, 2/1/14. https://doi.org/10.1117/12.2038265
Cha J, Paukert M, Bergles DE, Kang JU. Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8928. SPIE. 2014. 89282N https://doi.org/10.1117/12.2038265
Cha, Jaepyeong ; Paukert, Martin ; Bergles, Dwight E ; Kang, Jin U. / Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8928 SPIE, 2014.
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