FHIT gene alterations in esophageal cancer and ulcerative colitis (UC)

Tong Tong Zou, Junyi Lei, Ying Qiang Shi, Jing Yin, Suna Wang, Rhonda F. Souza, D. Kong, Yutaka Shimada, Kara N. Smolinski, Bruce D. Greenwald, John M. Abraham, Noam Harpaz, Stephen Meltzer

Research output: Contribution to journalArticle

Abstract

FHIT (fragile histidine triad gene), a candidate tumor suppressor gene, was recently identified and cloned at chromosome 3p14.2. Alterations of this gene have been reported in a number of primary human tumors, including colorectal, esophageal, gastric and lung carcinomas. However, some reports have found no abnormalities in this gene. We investigated a total of 63 primary esophageal tumors, nine esophageal cancer cell lines and 17 ulcerative colitis-associated neoplasms (UCANs) for alterations of FHIT. In 13 esophageal tumors, we employed overlapping reverse transcriptase-PCRs (RT-PCRs) to amplify and sequence the complete open reading frame of FHIT. One of 13 primary esophageal tumors analysed by RT-PCR expressed no detectable FHIT transcript; the remaining 12 expressed normal-sized transcripts with wild-type open reading frame sequences. In an additional 50 esophageal tumors, the polymorphic microsatellite loci D3S1300 and D3S1313 were used to evaluate loss of heterozygosity (LOH) at 3p14.2. Eleven of these 50 tumors showed LOH at one or both loci. In all these 11 tumors, genomic PCR and direct sequencing of FHIT exons 5-9 was performed. This analysis revealed that none of these 11 primary esophageal tumors contained any alterations in the FHIT open reading frame or adjacent intron sequences. Finally, among 17 UCANs, the in vitro synthesized protein (IVSP) assay detected no truncated protein products, nor were there any abnormalities in size or DNA sequence of FHIT RT-PCR products. However, in six of nine esophageal carcinoma cell lines, no FHIT RT-PCR product was detectable using either of the overlapping primer sets. Genomic PCR and direct sequencing of exons 5-9, also performed in these nine cell lines, revealed wild-type sequence in eight cell lines; however, one cell line contained no exon 5 PCR product. This cell line also lacked detectable FHIT transcript. These data suggest that the open reading frame of FHIT is not important in the development or progression of most primary esophageal carcinomas or UCANs, although lack of expression of the FHIT transcript may be common in esophageal cancer-derived cell lines. The possibility of an additional tumor suppressor gene at chromosome 3p14.2 remains to be evaluated.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalOncogene
Volume15
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Esophageal Neoplasms
Ulcerative Colitis
Histidine
Genes
Cell Line
Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Open Reading Frames
Exons
Loss of Heterozygosity
Tumor Suppressor Genes
Carcinoma
Polymerase Chain Reaction
Chromosomes
Microsatellite Repeats
Introns
Colorectal Neoplasms
Stomach
Proteins

Keywords

  • Esophageal carcinoma
  • FHIT
  • Mutation
  • Tumor suppressor gene
  • UC-associated neoplasms (UCANs)

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Zou, T. T., Lei, J., Shi, Y. Q., Yin, J., Wang, S., Souza, R. F., ... Meltzer, S. (1997). FHIT gene alterations in esophageal cancer and ulcerative colitis (UC). Oncogene, 15(1), 101-105.

FHIT gene alterations in esophageal cancer and ulcerative colitis (UC). / Zou, Tong Tong; Lei, Junyi; Shi, Ying Qiang; Yin, Jing; Wang, Suna; Souza, Rhonda F.; Kong, D.; Shimada, Yutaka; Smolinski, Kara N.; Greenwald, Bruce D.; Abraham, John M.; Harpaz, Noam; Meltzer, Stephen.

In: Oncogene, Vol. 15, No. 1, 1997, p. 101-105.

Research output: Contribution to journalArticle

Zou, TT, Lei, J, Shi, YQ, Yin, J, Wang, S, Souza, RF, Kong, D, Shimada, Y, Smolinski, KN, Greenwald, BD, Abraham, JM, Harpaz, N & Meltzer, S 1997, 'FHIT gene alterations in esophageal cancer and ulcerative colitis (UC)', Oncogene, vol. 15, no. 1, pp. 101-105.
Zou TT, Lei J, Shi YQ, Yin J, Wang S, Souza RF et al. FHIT gene alterations in esophageal cancer and ulcerative colitis (UC). Oncogene. 1997;15(1):101-105.
Zou, Tong Tong ; Lei, Junyi ; Shi, Ying Qiang ; Yin, Jing ; Wang, Suna ; Souza, Rhonda F. ; Kong, D. ; Shimada, Yutaka ; Smolinski, Kara N. ; Greenwald, Bruce D. ; Abraham, John M. ; Harpaz, Noam ; Meltzer, Stephen. / FHIT gene alterations in esophageal cancer and ulcerative colitis (UC). In: Oncogene. 1997 ; Vol. 15, No. 1. pp. 101-105.
@article{2d8eb86b22be4d8581b055f6015b81a4,
title = "FHIT gene alterations in esophageal cancer and ulcerative colitis (UC)",
abstract = "FHIT (fragile histidine triad gene), a candidate tumor suppressor gene, was recently identified and cloned at chromosome 3p14.2. Alterations of this gene have been reported in a number of primary human tumors, including colorectal, esophageal, gastric and lung carcinomas. However, some reports have found no abnormalities in this gene. We investigated a total of 63 primary esophageal tumors, nine esophageal cancer cell lines and 17 ulcerative colitis-associated neoplasms (UCANs) for alterations of FHIT. In 13 esophageal tumors, we employed overlapping reverse transcriptase-PCRs (RT-PCRs) to amplify and sequence the complete open reading frame of FHIT. One of 13 primary esophageal tumors analysed by RT-PCR expressed no detectable FHIT transcript; the remaining 12 expressed normal-sized transcripts with wild-type open reading frame sequences. In an additional 50 esophageal tumors, the polymorphic microsatellite loci D3S1300 and D3S1313 were used to evaluate loss of heterozygosity (LOH) at 3p14.2. Eleven of these 50 tumors showed LOH at one or both loci. In all these 11 tumors, genomic PCR and direct sequencing of FHIT exons 5-9 was performed. This analysis revealed that none of these 11 primary esophageal tumors contained any alterations in the FHIT open reading frame or adjacent intron sequences. Finally, among 17 UCANs, the in vitro synthesized protein (IVSP) assay detected no truncated protein products, nor were there any abnormalities in size or DNA sequence of FHIT RT-PCR products. However, in six of nine esophageal carcinoma cell lines, no FHIT RT-PCR product was detectable using either of the overlapping primer sets. Genomic PCR and direct sequencing of exons 5-9, also performed in these nine cell lines, revealed wild-type sequence in eight cell lines; however, one cell line contained no exon 5 PCR product. This cell line also lacked detectable FHIT transcript. These data suggest that the open reading frame of FHIT is not important in the development or progression of most primary esophageal carcinomas or UCANs, although lack of expression of the FHIT transcript may be common in esophageal cancer-derived cell lines. The possibility of an additional tumor suppressor gene at chromosome 3p14.2 remains to be evaluated.",
keywords = "Esophageal carcinoma, FHIT, Mutation, Tumor suppressor gene, UC-associated neoplasms (UCANs)",
author = "Zou, {Tong Tong} and Junyi Lei and Shi, {Ying Qiang} and Jing Yin and Suna Wang and Souza, {Rhonda F.} and D. Kong and Yutaka Shimada and Smolinski, {Kara N.} and Greenwald, {Bruce D.} and Abraham, {John M.} and Noam Harpaz and Stephen Meltzer",
year = "1997",
language = "English (US)",
volume = "15",
pages = "101--105",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - FHIT gene alterations in esophageal cancer and ulcerative colitis (UC)

AU - Zou, Tong Tong

AU - Lei, Junyi

AU - Shi, Ying Qiang

AU - Yin, Jing

AU - Wang, Suna

AU - Souza, Rhonda F.

AU - Kong, D.

AU - Shimada, Yutaka

AU - Smolinski, Kara N.

AU - Greenwald, Bruce D.

AU - Abraham, John M.

AU - Harpaz, Noam

AU - Meltzer, Stephen

PY - 1997

Y1 - 1997

N2 - FHIT (fragile histidine triad gene), a candidate tumor suppressor gene, was recently identified and cloned at chromosome 3p14.2. Alterations of this gene have been reported in a number of primary human tumors, including colorectal, esophageal, gastric and lung carcinomas. However, some reports have found no abnormalities in this gene. We investigated a total of 63 primary esophageal tumors, nine esophageal cancer cell lines and 17 ulcerative colitis-associated neoplasms (UCANs) for alterations of FHIT. In 13 esophageal tumors, we employed overlapping reverse transcriptase-PCRs (RT-PCRs) to amplify and sequence the complete open reading frame of FHIT. One of 13 primary esophageal tumors analysed by RT-PCR expressed no detectable FHIT transcript; the remaining 12 expressed normal-sized transcripts with wild-type open reading frame sequences. In an additional 50 esophageal tumors, the polymorphic microsatellite loci D3S1300 and D3S1313 were used to evaluate loss of heterozygosity (LOH) at 3p14.2. Eleven of these 50 tumors showed LOH at one or both loci. In all these 11 tumors, genomic PCR and direct sequencing of FHIT exons 5-9 was performed. This analysis revealed that none of these 11 primary esophageal tumors contained any alterations in the FHIT open reading frame or adjacent intron sequences. Finally, among 17 UCANs, the in vitro synthesized protein (IVSP) assay detected no truncated protein products, nor were there any abnormalities in size or DNA sequence of FHIT RT-PCR products. However, in six of nine esophageal carcinoma cell lines, no FHIT RT-PCR product was detectable using either of the overlapping primer sets. Genomic PCR and direct sequencing of exons 5-9, also performed in these nine cell lines, revealed wild-type sequence in eight cell lines; however, one cell line contained no exon 5 PCR product. This cell line also lacked detectable FHIT transcript. These data suggest that the open reading frame of FHIT is not important in the development or progression of most primary esophageal carcinomas or UCANs, although lack of expression of the FHIT transcript may be common in esophageal cancer-derived cell lines. The possibility of an additional tumor suppressor gene at chromosome 3p14.2 remains to be evaluated.

AB - FHIT (fragile histidine triad gene), a candidate tumor suppressor gene, was recently identified and cloned at chromosome 3p14.2. Alterations of this gene have been reported in a number of primary human tumors, including colorectal, esophageal, gastric and lung carcinomas. However, some reports have found no abnormalities in this gene. We investigated a total of 63 primary esophageal tumors, nine esophageal cancer cell lines and 17 ulcerative colitis-associated neoplasms (UCANs) for alterations of FHIT. In 13 esophageal tumors, we employed overlapping reverse transcriptase-PCRs (RT-PCRs) to amplify and sequence the complete open reading frame of FHIT. One of 13 primary esophageal tumors analysed by RT-PCR expressed no detectable FHIT transcript; the remaining 12 expressed normal-sized transcripts with wild-type open reading frame sequences. In an additional 50 esophageal tumors, the polymorphic microsatellite loci D3S1300 and D3S1313 were used to evaluate loss of heterozygosity (LOH) at 3p14.2. Eleven of these 50 tumors showed LOH at one or both loci. In all these 11 tumors, genomic PCR and direct sequencing of FHIT exons 5-9 was performed. This analysis revealed that none of these 11 primary esophageal tumors contained any alterations in the FHIT open reading frame or adjacent intron sequences. Finally, among 17 UCANs, the in vitro synthesized protein (IVSP) assay detected no truncated protein products, nor were there any abnormalities in size or DNA sequence of FHIT RT-PCR products. However, in six of nine esophageal carcinoma cell lines, no FHIT RT-PCR product was detectable using either of the overlapping primer sets. Genomic PCR and direct sequencing of exons 5-9, also performed in these nine cell lines, revealed wild-type sequence in eight cell lines; however, one cell line contained no exon 5 PCR product. This cell line also lacked detectable FHIT transcript. These data suggest that the open reading frame of FHIT is not important in the development or progression of most primary esophageal carcinomas or UCANs, although lack of expression of the FHIT transcript may be common in esophageal cancer-derived cell lines. The possibility of an additional tumor suppressor gene at chromosome 3p14.2 remains to be evaluated.

KW - Esophageal carcinoma

KW - FHIT

KW - Mutation

KW - Tumor suppressor gene

KW - UC-associated neoplasms (UCANs)

UR - http://www.scopus.com/inward/record.url?scp=8544262240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8544262240&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 101

EP - 105

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 1

ER -