Fever in the test tube--towards a human(e) pyrogen test.

Stephanie Schindler; Stefan Fennrich; Reto Crameri; Thomas W. Jungi; Thomas Montag; Thomas Hartung

Fever in the test tube--towards a human(e) pyrogen test.

Stephanie Schindler, Stefan Fennrich, Reto Crameri, Thomas W. Jungi, Thomas Montag, Thomas Hartung

Research output: Contribution to journal › Article › peer-review

Abstract

The human whole blood IL-1 test exploits the reaction of monocytes/macrophages for the detection of pyrogens: human whole blood taken from healthy volunteers is incubated in the presence of the test sample in any form, be it a solution, a powder or even solid material. Pyrogenic contaminations initiate the release of the "endogenous pyrogen" Interleukin-1beta determined by ELISA after incubation. In order to understand any differences between the pyrogenic activity in this test and the existing live rabbit test (species differences versus aberrant response of the particular blood sample), the rabbit whole blood test was developed. This approach could also help to avoid the use of putatively infectious human blood for pyrogen testing in vitro.

Original language	English (US)
Pages (from-to)	60-62
Number of pages	3
Journal	ALTEX : Alternativen zu Tierexperimenten
Volume	24 Spec No
State	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Medical Laboratory Technology

Cite this

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abstract = "The human whole blood IL-1 test exploits the reaction of monocytes/macrophages for the detection of pyrogens: human whole blood taken from healthy volunteers is incubated in the presence of the test sample in any form, be it a solution, a powder or even solid material. Pyrogenic contaminations initiate the release of the {"}endogenous pyrogen{"} Interleukin-1beta determined by ELISA after incubation. In order to understand any differences between the pyrogenic activity in this test and the existing live rabbit test (species differences versus aberrant response of the particular blood sample), the rabbit whole blood test was developed. This approach could also help to avoid the use of putatively infectious human blood for pyrogen testing in vitro.",

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AU - Fennrich, Stefan

AU - Crameri, Reto

AU - Jungi, Thomas W.

AU - Montag, Thomas

AU - Hartung, Thomas

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PY - 2007

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N2 - The human whole blood IL-1 test exploits the reaction of monocytes/macrophages for the detection of pyrogens: human whole blood taken from healthy volunteers is incubated in the presence of the test sample in any form, be it a solution, a powder or even solid material. Pyrogenic contaminations initiate the release of the "endogenous pyrogen" Interleukin-1beta determined by ELISA after incubation. In order to understand any differences between the pyrogenic activity in this test and the existing live rabbit test (species differences versus aberrant response of the particular blood sample), the rabbit whole blood test was developed. This approach could also help to avoid the use of putatively infectious human blood for pyrogen testing in vitro.

AB - The human whole blood IL-1 test exploits the reaction of monocytes/macrophages for the detection of pyrogens: human whole blood taken from healthy volunteers is incubated in the presence of the test sample in any form, be it a solution, a powder or even solid material. Pyrogenic contaminations initiate the release of the "endogenous pyrogen" Interleukin-1beta determined by ELISA after incubation. In order to understand any differences between the pyrogenic activity in this test and the existing live rabbit test (species differences versus aberrant response of the particular blood sample), the rabbit whole blood test was developed. This approach could also help to avoid the use of putatively infectious human blood for pyrogen testing in vitro.

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Fever in the test tube--towards a human(e) pyrogen test.

Abstract

ASJC Scopus subject areas

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