Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors

Differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell

George J Dover, T. Chan, F. Sieber

Research output: Contribution to journalArticle

Abstract

Single-cell microscopic immunodiffusion assays were used to determine the cellular mechanisms that regulate fetal hemoglobin (HbF) levels in cultures of primitive and late erythroid precursors obtained from human adult bone marrow. Two variables - the percentage of cells containing HbF (F cells) and the picograms (pg) of HbF/F cell - were assayed in cells derived from erythroid colony-forming units (CFU-E) and from erythroid burst-forming units (BFU-E) at 7 and 14 days in culture, respectively. The percentage of F cells among all nucleated cells from CFU-E-derived colonies (29.4% ± 12.5%, mean ± SD) was not significantly different (p = 0.2) from the percentage of F cells from BFU-E-derived bursts (37.3% ± 10.1%). Serial daily assays of all cells in cultures on days 3 through 7 and on day 14 revealed a marked increase in F cells between days 4 and 6 in culture. The average amount of HbF/F cell was less in CFU-E-derived F cells than in BFU-E-derived cells (3.5 ± 0.3 pg versus 6.2 ± 3.3 pg; p

Original languageEnglish (US)
Pages (from-to)1242-1246
Number of pages5
JournalBlood
Volume61
Issue number6
StatePublished - 1983

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Fetal Hemoglobin
Assays
Erythroid Precursor Cells
Bone
Immunodiffusion
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

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title = "Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors: Differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell",
abstract = "Single-cell microscopic immunodiffusion assays were used to determine the cellular mechanisms that regulate fetal hemoglobin (HbF) levels in cultures of primitive and late erythroid precursors obtained from human adult bone marrow. Two variables - the percentage of cells containing HbF (F cells) and the picograms (pg) of HbF/F cell - were assayed in cells derived from erythroid colony-forming units (CFU-E) and from erythroid burst-forming units (BFU-E) at 7 and 14 days in culture, respectively. The percentage of F cells among all nucleated cells from CFU-E-derived colonies (29.4{\%} ± 12.5{\%}, mean ± SD) was not significantly different (p = 0.2) from the percentage of F cells from BFU-E-derived bursts (37.3{\%} ± 10.1{\%}). Serial daily assays of all cells in cultures on days 3 through 7 and on day 14 revealed a marked increase in F cells between days 4 and 6 in culture. The average amount of HbF/F cell was less in CFU-E-derived F cells than in BFU-E-derived cells (3.5 ± 0.3 pg versus 6.2 ± 3.3 pg; p",
author = "Dover, {George J} and T. Chan and F. Sieber",
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language = "English (US)",
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pages = "1242--1246",
journal = "Blood",
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publisher = "American Society of Hematology",
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TY - JOUR

T1 - Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors

T2 - Differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell

AU - Dover, George J

AU - Chan, T.

AU - Sieber, F.

PY - 1983

Y1 - 1983

N2 - Single-cell microscopic immunodiffusion assays were used to determine the cellular mechanisms that regulate fetal hemoglobin (HbF) levels in cultures of primitive and late erythroid precursors obtained from human adult bone marrow. Two variables - the percentage of cells containing HbF (F cells) and the picograms (pg) of HbF/F cell - were assayed in cells derived from erythroid colony-forming units (CFU-E) and from erythroid burst-forming units (BFU-E) at 7 and 14 days in culture, respectively. The percentage of F cells among all nucleated cells from CFU-E-derived colonies (29.4% ± 12.5%, mean ± SD) was not significantly different (p = 0.2) from the percentage of F cells from BFU-E-derived bursts (37.3% ± 10.1%). Serial daily assays of all cells in cultures on days 3 through 7 and on day 14 revealed a marked increase in F cells between days 4 and 6 in culture. The average amount of HbF/F cell was less in CFU-E-derived F cells than in BFU-E-derived cells (3.5 ± 0.3 pg versus 6.2 ± 3.3 pg; p

AB - Single-cell microscopic immunodiffusion assays were used to determine the cellular mechanisms that regulate fetal hemoglobin (HbF) levels in cultures of primitive and late erythroid precursors obtained from human adult bone marrow. Two variables - the percentage of cells containing HbF (F cells) and the picograms (pg) of HbF/F cell - were assayed in cells derived from erythroid colony-forming units (CFU-E) and from erythroid burst-forming units (BFU-E) at 7 and 14 days in culture, respectively. The percentage of F cells among all nucleated cells from CFU-E-derived colonies (29.4% ± 12.5%, mean ± SD) was not significantly different (p = 0.2) from the percentage of F cells from BFU-E-derived bursts (37.3% ± 10.1%). Serial daily assays of all cells in cultures on days 3 through 7 and on day 14 revealed a marked increase in F cells between days 4 and 6 in culture. The average amount of HbF/F cell was less in CFU-E-derived F cells than in BFU-E-derived cells (3.5 ± 0.3 pg versus 6.2 ± 3.3 pg; p

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