Fes mediates the IL-4 activation of insulin receptor substrate-2 and cellular proliferation

H. Jiang, K. Foltenyi, M. Kashiwada, L. Donahue, B. Vuong, B. Hehn, P. Rothman

Research output: Contribution to journalArticle

Abstract

Although Jak kinases are essential for initiating cytokine signaling, the role of other nonreceptor tyrosine kinases in this process remains unclear. We have examined the role of Fes in IL-4 signaling. Examination of Jak1-deficient cell lines demonstrates that Jak1 is required for the activation of Fes by IL-4. Experiments studying signaling molecules activated by IL-4 receptor suggest that IL-4 signaling can be subdivided into Fes-dependent and Fes-independent pathways. Overexpression of kinase-inactive Fes blocks the IL-4 activation of insulin receptor substrate-2, but not STAT6. Fes appears to be a downstream kinase from Jak1/Jak3 in this process. Further examination of downstream signaling demonstrates that kinase-inactive Fes inhibits the recruitment of phosphoinositide 3-kinase to the activated IL-4 receptor complex and decreases the activation of p70S6k kinase in response to IL-4. This inhibition correlates with a decrease in IL-4-induced proliferation. In contrast, mutant Fes does not inhibit the activation of Akt by IL-4. These data demonstrate that signaling pathways activated by IL-4 require different tyrosine kinases. This differential requirement predicts that specific kinase inhibitors may permit the disruption of specific IL-4-induced functions.

Original languageEnglish (US)
Pages (from-to)2627-2634
Number of pages8
JournalJournal of Immunology
Volume166
Issue number4
DOIs
StatePublished - Feb 15 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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