Female mouse germ cells form synchronously dividing cysts

Melissa E. Pepling, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Oocytes from many invertebrates initiate development within distinctive cysts of interconnected cells, which are formed through synchronous divisions of a progenitor cell. Recently, processes underlying cyst formation have been extensively characterized at the molecular level in Drosophila. Defects in this process cause sterility in female flies. Early female mouse germ cells are organized as cell clusters as well, but it is uncertain whether these groups are similar to the cysts of invertebrates. We find that mouse germ cells are connected by intercellular bridges in the ovaries of 11.5 to 17.5 days postcoitum embryos; microtubules and organelles have been observed within these bridges. Confocal microscopy shows that cells within mouse clusters divide synchronously and frequently correspond in number to powers of two. Thus, female mouse germ cell clusters exhibit key characteristics of invertebrate germline cysts indicating that the process of germline cyst formation is conserved in the mouse.

Original languageEnglish (US)
Pages (from-to)3323-3328
Number of pages6
JournalDevelopment
Volume125
Issue number17
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Germ Cells
Cysts
Invertebrates
Female Infertility
Confocal Microscopy
Microtubules
Diptera
Organelles
Drosophila
Oocytes
Ovary
Stem Cells
Embryonic Structures

Keywords

  • Cyst
  • Mouse germ cell
  • Oogenesis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Pepling, M. E., & Spradling, A. C. (1998). Female mouse germ cells form synchronously dividing cysts. Development, 125(17), 3323-3328.

Female mouse germ cells form synchronously dividing cysts. / Pepling, Melissa E.; Spradling, Allan C.

In: Development, Vol. 125, No. 17, 09.1998, p. 3323-3328.

Research output: Contribution to journalArticle

Pepling, ME & Spradling, AC 1998, 'Female mouse germ cells form synchronously dividing cysts', Development, vol. 125, no. 17, pp. 3323-3328.
Pepling ME, Spradling AC. Female mouse germ cells form synchronously dividing cysts. Development. 1998 Sep;125(17):3323-3328.
Pepling, Melissa E. ; Spradling, Allan C. / Female mouse germ cells form synchronously dividing cysts. In: Development. 1998 ; Vol. 125, No. 17. pp. 3323-3328.
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