Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial follicles

Lei Lei, Allan C. Spradling

Research output: Contribution to journalArticlepeer-review

Abstract

Whether or not mammalian females generate new oocytes during adulthood from germ-line stem cells to sustain the ovarian follicle pool has recently generated controversy.We used a sensitive lineagelabelingsystem to determinewhether stem cells are needed in female adult mice to compensate for follicular losses and to directly identify active germ-line stemcells. Primordial follicles generated during fetal life are highly stable, with a half-life during adulthood of 10mo, and thus are sufficient to sustain adult oogenesis without a source of renewal. Moreover, in normal mice or following germ-cell depletion with Busulfan, only stable, single oocytes are lineage-labeled, rather than cell clusters indicative of newoocyte formation. Even one germline stem cell division per 2 wk would have been detected by our method, based on the kinetics of fetal follicle formation. Thus, adult femalemice neither require nor contain active germ-line stem cells or produce new oocytes in vivo.

Original languageEnglish (US)
Pages (from-to)8585-8590
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number21
DOIs
StatePublished - May 21 2013
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial follicles'. Together they form a unique fingerprint.

Cite this