Feedback control of mitosis in budding yeast

Rong Li, Andrew W. Murray

Research output: Contribution to journalArticle

Abstract

We have investigated the feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. We isolated budding yeast mutants sensitive to the anti-microtubule drug benomyl. Mitotic arrest-deficient (mad) mutants are the subclass of benomyl-sensitive mutants in which the completion of mitosis is not delayed in the presence of benomyl and that die as a consequence of their premature exit from mitosis. A number of properties of the mad mutants indicate that they are defective in the feedback control over the exit from mitosis: their killing by benomyl requires passage through mitosis; their benomyl sensitivity can be suppressed by an independent method for delaying the exit from mitosis; they have normal microtubules; and they have increased frequencies of chromosome loss. We cloned MAD2, which encodes a putative calcium-binding protein whose disruption is lethal. We discuss the role of feedback controls in coordinating events in the cell cycle.

Original languageEnglish (US)
Pages (from-to)519-531
Number of pages13
JournalCell
Volume66
Issue number3
StatePublished - 1991
Externally publishedYes

Fingerprint

Benomyl
Saccharomycetales
Mitosis
Yeast
Feedback control
Microtubules
Calcium-Binding Proteins
Chromosomes
Cells
Cell Cycle
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Li, R., & Murray, A. W. (1991). Feedback control of mitosis in budding yeast. Cell, 66(3), 519-531.

Feedback control of mitosis in budding yeast. / Li, Rong; Murray, Andrew W.

In: Cell, Vol. 66, No. 3, 1991, p. 519-531.

Research output: Contribution to journalArticle

Li, R & Murray, AW 1991, 'Feedback control of mitosis in budding yeast', Cell, vol. 66, no. 3, pp. 519-531.
Li R, Murray AW. Feedback control of mitosis in budding yeast. Cell. 1991;66(3):519-531.
Li, Rong ; Murray, Andrew W. / Feedback control of mitosis in budding yeast. In: Cell. 1991 ; Vol. 66, No. 3. pp. 519-531.
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