TY - JOUR
T1 - Fecal excretion of greek strains of hepatitis a virus in patients with hepatitis a and in experimentally infected chimpanzees
AU - Tassopoulos, Nicolaos C.
AU - Papaevangelou, George J.
AU - Ticehurst, John R.
AU - Purcell, Robert H.
N1 - Funding Information:
This study was supported by a grant from the Ministry of Health and Welfare and from the Ministry of Research and Technology of Greece. Dr. Tassopoulos was a Visiting Scientist, Fogarty International Center. National Institutes of Health, Bethesda, Maryland.
PY - 1986/8/1
Y1 - 1986/8/1
N2 - The presence of hepatitis A virus (HAV) in stool samples was determined in 36 children (mean age, 8.9 years) and 38 adults (mean age, 19.9 years) with acute type A hepatitis. Three stool samples, taken on admission and thereafter at three-to-five-day intervals, were collected from each patient. The first day of dark urine was considered to be the onset of illness. Molecular hybridization of cloned HAV cDNA to fecal extracts was used to detect HAV RNA; radioimmunoassay was used to detect HAV antigen. In all of the samples tested, HAV RNA was detected significantly more frequently than HAV antigen (28.4% vs. 8.1%, P <.001). HAV RNA was detected with equal frequency in both children and adults during the first week of illness. However, HAV RNA was detected more frequently in children than in adults during the second week of illness (45.7% vs. 18.9%, P <.05). Among patients with HAV RNA, detection in multiple samples was more frequent in children than in adults (38.9% vs. 7.9%, P <.01), especially among males.
AB - The presence of hepatitis A virus (HAV) in stool samples was determined in 36 children (mean age, 8.9 years) and 38 adults (mean age, 19.9 years) with acute type A hepatitis. Three stool samples, taken on admission and thereafter at three-to-five-day intervals, were collected from each patient. The first day of dark urine was considered to be the onset of illness. Molecular hybridization of cloned HAV cDNA to fecal extracts was used to detect HAV RNA; radioimmunoassay was used to detect HAV antigen. In all of the samples tested, HAV RNA was detected significantly more frequently than HAV antigen (28.4% vs. 8.1%, P <.001). HAV RNA was detected with equal frequency in both children and adults during the first week of illness. However, HAV RNA was detected more frequently in children than in adults during the second week of illness (45.7% vs. 18.9%, P <.05). Among patients with HAV RNA, detection in multiple samples was more frequent in children than in adults (38.9% vs. 7.9%, P <.01), especially among males.
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U2 - 10.1093/infdis/154.2.231
DO - 10.1093/infdis/154.2.231
M3 - Article
C2 - 3014009
AN - SCOPUS:0022550423
SN - 0022-1899
VL - 154
SP - 231
EP - 237
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -