Feasibility of blood flow measurement by externally controlled dye delivery

R. C. Zeimer, B. Khoobehi, G. Peyman, M. R. Niesman, R. L. Magin

Research output: Contribution to journalArticlepeer-review


We are developing a new method of delivering substances locally and repeatedly in the retinal vasculature under external control. This delivery system is based on encapsulating the substance in heat-sensitive liposomes, which are injected intravenously and lysed by a heat pulse delivered by a laser. The feasibility of using this system with dyes and creating a sharp dye front was tested in vitro and in vivo. The results indicate that the background fluorescence of intact liposomes is minimal but in contrast a dramatic increase in fluorescence is achieved where the dye is released. In vivo tests indicated that only the selected vascular branch fluoresced. Moreover, a sharp dye front could be obtained repeatedly and preserved over significant distances. The presence of a sharp dye front allowed measurements, in vivo, of blood velocity which correlated well (r = 0.985, P<0.001) with the average blood velocity values calculated from the known flow rate.

Original languageEnglish (US)
Pages (from-to)660-667
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Issue number4
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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