Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients

Young Wang, Francis P. Kuhajda, Ji Nong Li, Ellen S. Pizer, Wan Fang Han, Lori J Sokoll, Daniel Wan-Yui Chan

Research output: Contribution to journalArticle

Abstract

Fatty acid synthase (FAS) is selectively expressed in certain human cancers, including carcinoma of the breast, prostate, colon, ovary, and endometrium, compared to normal human tissues and therefore is a putative tumor marker. In this study, we found FAS concentrations were elevated in cell culture supernatants during cell growth in two human breast cancer cell lines but not other cancer cell lines. A quantitative enzyme-linked immunosorbent assay and Western blot analysis were employed in this study. In addition, serum FAS levels were significantly higher in breast cancer patients with different clinical stages (Stage II: 0.59 ± 0.09 units/l, Stage III: 0.79 ± 0.13 units/l, and Stage IV: 1.39 ± 0.35 units/l) compared with healthy subjects (0.27 ± 0.02 units/l, P <0.05). Taken together, our data suggest that FAS expression may be a useful tumor marker for breast cancer and play a role in assessing cancer virulence.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalCancer Letters
Volume167
Issue number1
DOIs
StatePublished - Jun 10 2001

Fingerprint

Fatty Acid Synthases
Cell Culture Techniques
Breast Neoplasms
Tumor Biomarkers
Cell Line
Neoplasms
Endometrium
Virulence
Prostate
Ovary
Healthy Volunteers
Colon
Western Blotting
Enzyme-Linked Immunosorbent Assay
Growth
Serum

Keywords

  • Enzyme
  • Fatty acid synthase
  • Immunoassay
  • Tumor marker
  • Western blot

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients. / Wang, Young; Kuhajda, Francis P.; Li, Ji Nong; Pizer, Ellen S.; Han, Wan Fang; Sokoll, Lori J; Chan, Daniel Wan-Yui.

In: Cancer Letters, Vol. 167, No. 1, 10.06.2001, p. 99-104.

Research output: Contribution to journalArticle

Wang, Young ; Kuhajda, Francis P. ; Li, Ji Nong ; Pizer, Ellen S. ; Han, Wan Fang ; Sokoll, Lori J ; Chan, Daniel Wan-Yui. / Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients. In: Cancer Letters. 2001 ; Vol. 167, No. 1. pp. 99-104.
@article{affd51dbc9f2431f904cf1a1c6350e26,
title = "Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients",
abstract = "Fatty acid synthase (FAS) is selectively expressed in certain human cancers, including carcinoma of the breast, prostate, colon, ovary, and endometrium, compared to normal human tissues and therefore is a putative tumor marker. In this study, we found FAS concentrations were elevated in cell culture supernatants during cell growth in two human breast cancer cell lines but not other cancer cell lines. A quantitative enzyme-linked immunosorbent assay and Western blot analysis were employed in this study. In addition, serum FAS levels were significantly higher in breast cancer patients with different clinical stages (Stage II: 0.59 ± 0.09 units/l, Stage III: 0.79 ± 0.13 units/l, and Stage IV: 1.39 ± 0.35 units/l) compared with healthy subjects (0.27 ± 0.02 units/l, P <0.05). Taken together, our data suggest that FAS expression may be a useful tumor marker for breast cancer and play a role in assessing cancer virulence.",
keywords = "Enzyme, Fatty acid synthase, Immunoassay, Tumor marker, Western blot",
author = "Young Wang and Kuhajda, {Francis P.} and Li, {Ji Nong} and Pizer, {Ellen S.} and Han, {Wan Fang} and Sokoll, {Lori J} and Chan, {Daniel Wan-Yui}",
year = "2001",
month = "6",
day = "10",
doi = "10.1016/S0304-3835(01)00464-5",
language = "English (US)",
volume = "167",
pages = "99--104",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Fatty acid synthase (FAS) expression in human breast cancer cell culture supernatants and in breast cancer patients

AU - Wang, Young

AU - Kuhajda, Francis P.

AU - Li, Ji Nong

AU - Pizer, Ellen S.

AU - Han, Wan Fang

AU - Sokoll, Lori J

AU - Chan, Daniel Wan-Yui

PY - 2001/6/10

Y1 - 2001/6/10

N2 - Fatty acid synthase (FAS) is selectively expressed in certain human cancers, including carcinoma of the breast, prostate, colon, ovary, and endometrium, compared to normal human tissues and therefore is a putative tumor marker. In this study, we found FAS concentrations were elevated in cell culture supernatants during cell growth in two human breast cancer cell lines but not other cancer cell lines. A quantitative enzyme-linked immunosorbent assay and Western blot analysis were employed in this study. In addition, serum FAS levels were significantly higher in breast cancer patients with different clinical stages (Stage II: 0.59 ± 0.09 units/l, Stage III: 0.79 ± 0.13 units/l, and Stage IV: 1.39 ± 0.35 units/l) compared with healthy subjects (0.27 ± 0.02 units/l, P <0.05). Taken together, our data suggest that FAS expression may be a useful tumor marker for breast cancer and play a role in assessing cancer virulence.

AB - Fatty acid synthase (FAS) is selectively expressed in certain human cancers, including carcinoma of the breast, prostate, colon, ovary, and endometrium, compared to normal human tissues and therefore is a putative tumor marker. In this study, we found FAS concentrations were elevated in cell culture supernatants during cell growth in two human breast cancer cell lines but not other cancer cell lines. A quantitative enzyme-linked immunosorbent assay and Western blot analysis were employed in this study. In addition, serum FAS levels were significantly higher in breast cancer patients with different clinical stages (Stage II: 0.59 ± 0.09 units/l, Stage III: 0.79 ± 0.13 units/l, and Stage IV: 1.39 ± 0.35 units/l) compared with healthy subjects (0.27 ± 0.02 units/l, P <0.05). Taken together, our data suggest that FAS expression may be a useful tumor marker for breast cancer and play a role in assessing cancer virulence.

KW - Enzyme

KW - Fatty acid synthase

KW - Immunoassay

KW - Tumor marker

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=0035838120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035838120&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(01)00464-5

DO - 10.1016/S0304-3835(01)00464-5

M3 - Article

C2 - 11323104

AN - SCOPUS:0035838120

VL - 167

SP - 99

EP - 104

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -