Fatty acid remodeling: A novel reaction sequence in the biosynthesis of trypanosome glycosyl phosphatidylinositol membrane anchors

Wayne J. Masterson, Jayne Raper, Tamara L. Doering, Gerald W. Hart, Paul T. Englund

Research output: Contribution to journalArticle

Abstract

The trypanosome variant surface glycoprotein (VSG) is anchored to the plasma membrane via a glycosyl phosphatidylinositol (GPI). The GPI is synthesized as a precursor, glycolipid A, that is subsequently linked to the VSG polypeptide. The VSG anchor is unusual, compared with anchors in other cell types, in that its fatty acid moieties are exclusively myristic acid. To investigate the mechanism for myristate specificity we used a cell-free system for GPI biosynthesis. One product of this system, glycolipid A′, is indistinguishable from glycolipid A except that its fatty acids are more hydrophobic than myristate. Glycolipid A′ is converted to glycolipid A through highly specific fatty acid remodeling reactions involving deacylation and subsequent reacylation with myristate. Therefore, myristoylation occurs in the final phase of trypanosome GPI biosynthesis.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalCell
Volume62
Issue number1
DOIs
StatePublished - Jul 13 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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