TY - JOUR
T1 - Fate-restricted neural progenitors in the mammalian cerebral cortex
AU - Franco, Santos J.
AU - Gil-Sanz, Cristina
AU - Martinez-Garay, Isabel
AU - Espinosa, Ana
AU - Harkins-Perry, Sarah R.
AU - Ramos, Cynthia
AU - Müller, Ulrich
PY - 2012/8/10
Y1 - 2012/8/10
N2 - During development of the mammalian cerebral cortex, radial glial cells (RGCs) generate layer-specific subtypes of excitatory neurons in a defined temporal sequence, in which lower-layer neurons are formed before upper-layer neurons. It has been proposed that neuronal subtype fate is determined by birthdate through progressive restriction of the neurogenic potential of a common RGC progenitor. Here, we demonstrate that the murine cerebral cortex contains RGC sublineages with distinct fate potentials. Using in vivo genetic fate mapping and in vitro clonal analysis, we identified an RGC lineage that is intrinsically specified to generate only upper-layer neurons, independently of niche and birthdate. Because upper cortical layers were expanded during primate evolution, amplification of this RGC pool may have facilitated human brain evolution.
AB - During development of the mammalian cerebral cortex, radial glial cells (RGCs) generate layer-specific subtypes of excitatory neurons in a defined temporal sequence, in which lower-layer neurons are formed before upper-layer neurons. It has been proposed that neuronal subtype fate is determined by birthdate through progressive restriction of the neurogenic potential of a common RGC progenitor. Here, we demonstrate that the murine cerebral cortex contains RGC sublineages with distinct fate potentials. Using in vivo genetic fate mapping and in vitro clonal analysis, we identified an RGC lineage that is intrinsically specified to generate only upper-layer neurons, independently of niche and birthdate. Because upper cortical layers were expanded during primate evolution, amplification of this RGC pool may have facilitated human brain evolution.
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U2 - 10.1126/science.1223616
DO - 10.1126/science.1223616
M3 - Article
C2 - 22879516
AN - SCOPUS:84864861869
VL - 337
SP - 746
EP - 749
JO - Science
JF - Science
SN - 0036-8075
IS - 6095
ER -