Fatal herpesvirus hemorrhagic disease in wild and orphan Asian elephants in Southern India

Arun Zachariah, Jianchao Zong, Simon Y. Long, Erin M. Latimer, Sarah Y. Heaggans, Laura K. Richman, Gary Selwyn Hayward

Research output: Contribution to journalArticle

Abstract

Up to 65% of deaths of young Asian elephants (Elephas maximus) between 3 mo and 15 yr of age in Europe and North America over the past 20 yr have been attributed to hemorrhagic disease associated with a novel DNA virus called elephant endotheliotropic herpesvirus (EEHV). To evaluate the potential role of EEHV in suspected cases of a similar lethal acute hemorrhagic disease occurring in southern India, we studied pathologic tissue samples collected from field necropsies. Nine cases among both orphaned camp and wild Asian elephants were identified by diagnostic PCR. These were subjected to detailed gene subtype DNA sequencing at multiple PCR loci, which revealed seven distinct strains of EEHV1A and one of EEHV1B. Two orphan calves that died within 3 days of one another at the same training camp had identical EEHV1A DNA sequences, indicating a common epidemiologic source. However, the high level of EEHV1 subtype genetic diversity found among the other Indian strains matches that among over 30 EEHV1 strains that have been evaluated from Europe and North America. These results argue against the previous suggestions that this is just a disease of captive elephants and that the EEHV1 virus has crossed recently from African elephant (Loxodonta africana) hosts to Asian elephants. Instead, both the virus and the disease are evidently widespread in Asia and, despite the disease severity, Asian elephants appear to be the ancient endogenous hosts of both EEHV1A and EEHV1B.

Original languageEnglish (US)
Pages (from-to)381-393
Number of pages13
JournalJournal of Wildlife Diseases
Volume49
Issue number2
DOIs
StatePublished - Apr 1 2013

Fingerprint

orphan
Elephas maximus
elephant
Elephantid herpesvirus 1
India
Loxodonta africana
virus
DNA viruses
viruses
DNA
Elephantidae
lethal genes
disease severity
necropsy
sequence analysis
calves
death
nucleotide sequences
genetic variation
loci

Keywords

  • Calves
  • EEHV1A
  • EEHV1B
  • Elephas maximus
  • Orphans
  • Proboscivirus
  • Wild

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Fatal herpesvirus hemorrhagic disease in wild and orphan Asian elephants in Southern India. / Zachariah, Arun; Zong, Jianchao; Long, Simon Y.; Latimer, Erin M.; Heaggans, Sarah Y.; Richman, Laura K.; Hayward, Gary Selwyn.

In: Journal of Wildlife Diseases, Vol. 49, No. 2, 01.04.2013, p. 381-393.

Research output: Contribution to journalArticle

Zachariah, Arun ; Zong, Jianchao ; Long, Simon Y. ; Latimer, Erin M. ; Heaggans, Sarah Y. ; Richman, Laura K. ; Hayward, Gary Selwyn. / Fatal herpesvirus hemorrhagic disease in wild and orphan Asian elephants in Southern India. In: Journal of Wildlife Diseases. 2013 ; Vol. 49, No. 2. pp. 381-393.
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