Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology

Aya Abdalla, Alyssa West, Yunju Jin, Rachel A. Saylor, Beidi Qiang, Edsel Peña, David J. Linden, H. Frederik Nijhout, Michael C. Reed, Janet Best, Parastoo Hashemi

Research output: Contribution to journalArticle

Abstract

It is important to monitor serotonin neurochemistry in the context of brain disorders. Specifically, a better understanding of biophysical alterations and associated biochemical functionality within subregions of the brain will enable better of understanding of diseases such as depression. Fast voltammetric tools at carbon fiber microelectrodes provide an opportunity to make direct evoked and ambient serotonin measurements in vivo in mice. In this study, we characterize novel stimulation and measurement circuitries for serotonin analyses in brain regions relevant to psychiatric disease. Evoked and ambient serotonin in these brain areas, the CA2 region of the hippocampus and the medial prefrontal cortex, are compared to ambient and evoked serotonin in the substantia nigra pars reticulata, an area well established previously for serotonin measurements with fast voltammetry. Stimulation of a common axonal location evoked serotonin in all three brain regions. Differences are observed in the serotonin release and reuptake profiles between these three brain areas which we hypothesize to arise from tissue physiology heterogeneity around the carbon fiber microelectrodes. We validate this hypothesis mathematically and via confocal imaging. We thereby show that fast voltammetric methods can provide accurate information about local physiology and highlight implications for chemical mapping. (Figure presented.). Cover Image for this issue: doi: 10.1111/jnc.14739.

Original languageEnglish (US)
Pages (from-to)33-50
Number of pages18
JournalJournal of Neurochemistry
Volume153
Issue number1
DOIs
StatePublished - Apr 1 2020

Keywords

  • FSCV
  • confocal microscopy
  • hippocampus
  • medial forebrain bundle
  • prefrontal cortex
  • substantia nigra

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology'. Together they form a unique fingerprint.

  • Cite this

    Abdalla, A., West, A., Jin, Y., Saylor, R. A., Qiang, B., Peña, E., Linden, D. J., Nijhout, H. F., Reed, M. C., Best, J., & Hashemi, P. (2020). Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology. Journal of Neurochemistry, 153(1), 33-50. https://doi.org/10.1111/jnc.14854