Fast assignment of 15N-HSQC peaks using high-resolution 3D HNcocaNH experiments with non-uniform sampling

Zhen Yu J. Sun, Dominique P. Frueh, Philipp Selenko, Jeffrey C. Hoch, Gerhard Wagner

Research output: Contribution to journalArticlepeer-review

Abstract

We describe an efficient NMR triple resonance approach for fast assignment of backbone amide resonance peaks in the 15N-HSQC spectrum. The exceptionally high resolutions achieved in the 3D HncocaNH and hNcocaNH experiments together with non-uniform sampling facilitate error-free sequential connection of backbone amides. Data required for the complete backbone amide assignment of the 56-residue protein GB1 domain were obtained in 14 h. Data analysis was vastly streamlined using a 'backbone NH walk' method to determine sequential connectivities without the need for 13C chemical shifts comparison. Amino acid residues in the sequentially connected NH chains are classified into two groups by a simple variation of the NMR pulse sequence, and the resulting 'ZeBra' stripe patterns are useful for mapping these chains to the protein sequence. In addition to resolving ambiguous assignments derived from conventional backbone experiments, this approach can be employed to rapidly assign small proteins or flexible regions in larger proteins, and to transfer assignments to mutant proteins or proteins in different ligand-binding states.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalJournal of Biomolecular NMR
Volume33
Issue number1
DOIs
StatePublished - Sep 2005
Externally publishedYes

Keywords

  • HNcocaNH
  • Maximum entropy reconstruction
  • Non-uniform sampling
  • Sequential assignment
  • Triplere sonance experiment

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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