TY - JOUR
T1 - Factors governing the mass loading of aerosolized carbon black particles with acid sulfates, inhalation exposure, and alveolar macrophage phagocytic function
AU - Hemenway, David R.
AU - Clarke, Robert
AU - Frank, Robert
AU - Jakab, George J.
N1 - Funding Information:
This study was supported in part by grant P30 ES03819 from the National Institute of Environmental Health Sciences (NIEHS) Center and NIEHS training grant 07141 for support of R. Clarke. The authors thank Emeka lfedigbo for his technical assistance on the inhalation exposure system.
PY - 1996/9
Y1 - 1996/9
N2 - A flow-past nose-only inhalation system is described for the coexposure of animals to carbon black aerosols (CBA) and sulfur dioxide (SO2) at varying relative humidities (RH). The conversion of SO2 to sulfate (SO4/2-) on the CBA, at a fixed aerosol concentration, was dependent on RH and SO2 concentration. The effect of the aerosol gas mixture on alveolar macrophage (AM) phagocytosis was assessed 3 days following nose-only exposure of mice for 4 h. Exposure to 10 mg/m3 CBA alone at low RH (10%) and high RH (85%), to 10 ppm SO2 alone at both RH, and to the mixture at low RH had no effect on AM phagocytosis. In contrast, AM phagocytosis was significantly suppressed following coexposure at 85% RH, the only circumstance in which significant chemisorption of the gas and oxidation to SO4/2- occurred. The results suggest that fine carbon particles are an effective vector for the delivery of toxic amounts of SO4/2- to the periphery of the lung under conditions of elevated RH.
AB - A flow-past nose-only inhalation system is described for the coexposure of animals to carbon black aerosols (CBA) and sulfur dioxide (SO2) at varying relative humidities (RH). The conversion of SO2 to sulfate (SO4/2-) on the CBA, at a fixed aerosol concentration, was dependent on RH and SO2 concentration. The effect of the aerosol gas mixture on alveolar macrophage (AM) phagocytosis was assessed 3 days following nose-only exposure of mice for 4 h. Exposure to 10 mg/m3 CBA alone at low RH (10%) and high RH (85%), to 10 ppm SO2 alone at both RH, and to the mixture at low RH had no effect on AM phagocytosis. In contrast, AM phagocytosis was significantly suppressed following coexposure at 85% RH, the only circumstance in which significant chemisorption of the gas and oxidation to SO4/2- occurred. The results suggest that fine carbon particles are an effective vector for the delivery of toxic amounts of SO4/2- to the periphery of the lung under conditions of elevated RH.
UR - http://www.scopus.com/inward/record.url?scp=0030003327&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030003327&partnerID=8YFLogxK
U2 - 10.3109/08958379609005447
DO - 10.3109/08958379609005447
M3 - Article
AN - SCOPUS:0030003327
SN - 0895-8378
VL - 8
SP - 679
EP - 694
JO - Inhalation Toxicology
JF - Inhalation Toxicology
IS - 7
ER -