Factors associated with incorrect identification of recent HIV infection using the BED capture immunoassay

Oliver B. Laeyendecker, Ron Brookmeyer, Amy E. Oliver, Caroline E. Mullis, Kevin Eaton, Amy C. Mueller, Lisa Paula Jacobson, Joseph Bernard Margolick, Joelle Brown, Charles R. Rinaldo, Thomas C Quinn, Susan Eshleman

Research output: Contribution to journalArticle

Abstract

The BED capture enzyme immunoassay (BED-CEIA) was developed for estimating HIV incidence from cross-sectional data. This assay misclassifies some individuals with nonrecent HIV infection as recently infected, leading to overestimation of HIV incidence. We analyzed factors associated with misclassification by the BED-CEIA. We analyzed samples from 383 men who were diagnosed with HIV infection less than 1 year after a negative HIV test (Multicenter AIDS Cohort Study). Samples were collected 2-8 years after HIV seroconversion, which was defined as the midpoint between the last negative and first positive HIV test. Samples were analyzed using the BED-CEIA with a cutoff of OD-n ≤0.8 for recent infection. Logistic regression was used to identify factors associated with misclassification. Ninety-one (15.1%) of 603 samples were misclassified. In multivariate models, misclassification was independently associated with highly active antiretroviral treatment (HAART) for >2 years, HIV RNA 3; adjusted odds ratios (OR) and 95% confidence intervals (CI) were 4.72 (1.35-16.5), 3.96 (1.53-10.3), 6.85 (2.71-17.4), and 11.5 (3.64-36.0), respectively. Among 220 men with paired samples, misclassification 2-4 years after seroconversion was significantly associated with misclassification 6-8 years after seroconversion [adjusted OR: 25.8 (95% CI: 8.17-81.5), p2 years of HAART were significantly associated with misclassification using the BED-CEIA. Some men were persistently misclassified as recently infected up to 8 years after HIV seroconversion.

Original languageEnglish (US)
Pages (from-to)816-822
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume28
Issue number8
DOIs
StatePublished - Aug 1 2012

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Immunoenzyme Techniques
Immunoassay
HIV Infections
HIV
HIV Seropositivity
Odds Ratio
Confidence Intervals
HIV-2
Incidence
Acquired Immunodeficiency Syndrome
Cohort Studies
Logistic Models
RNA
Therapeutics
Infection
Seroconversion

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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Factors associated with incorrect identification of recent HIV infection using the BED capture immunoassay. / Laeyendecker, Oliver B.; Brookmeyer, Ron; Oliver, Amy E.; Mullis, Caroline E.; Eaton, Kevin; Mueller, Amy C.; Jacobson, Lisa Paula; Margolick, Joseph Bernard; Brown, Joelle; Rinaldo, Charles R.; Quinn, Thomas C; Eshleman, Susan.

In: AIDS Research and Human Retroviruses, Vol. 28, No. 8, 01.08.2012, p. 816-822.

Research output: Contribution to journalArticle

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