Factors affecting transfer of experimental autoimmune thyroiditis in rats

N. R. Rose, M. F. Molotchnikoff, F. J. Twarog

Research output: Contribution to journalArticlepeer-review

Abstract

Actively induced experimental autoimmune thyroiditis in inbred Lewis rats was comparable using standard Freund's complete adjuvant (FCA) and Perrin's modification of FCA. However, adoptively transferred disease using lymph node cells from rats immunized with Perrin's FCA was significantly more severe. With this adjuvant, and pertussis vaccine as coadjuvant, transfer was uniformly successful when at least 480 x 106 lymph node cells were taken 10 days after immunization and recipients were killed 3 days after transfer. Lymphocytic infiltrates were seen in recipient thyroids as early as 18 hr after transfer. Whole body irradiation of the recipients at 550 r reduced the severity of transferred disease. The frequency and severity of lesions were higher when the lymph node cells were first incubated with low doses of antigen. Thymectomy of the recipients decreased the severity of transferred disease. Under the conditions tested, transfer of disease could not be accomplished by antiserum alone, even using thyroidectomized donors. Administration of an early immune serum with sensitized lymph node cells significantly depressed the severity of transferred disease, while a late antiserum increased it.

Original languageEnglish (US)
Pages (from-to)859-870
Number of pages12
JournalImmunology
Volume24
Issue number5
StatePublished - Dec 1 1973

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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