Facilitated molecular typing of shigella isolates using ERIC-PCR

Margaret Kosek, Pablo Penataro Yori, Robert H. Gilman, Henry Vela, Maribel Paredes Olortegui, Cesar Banda Chavez, Maritza Calderon, Juan Perez Bao, Eric Hall, Ryan Maves, Rosa Burga, Graciela Meza Sanchez

Research output: Contribution to journalArticlepeer-review

Abstract

To evaluate the performance of enterobacterial repetitive intergenic sequence-based polymerase chain reaction (ERIC-PCR) typing versus the current standard for the typing of Shigella pulsed gel electrophoresis (PFGE), we typed 116 Shigella isolates from a village in an endemic setting over a 20-month period using both methods. PFGE identified 37 pulse types and had a discrimination index of 0.925 (95% confidence interval = 0.830-1.00), whereas ERIC-PCR identified 42 types and had a discrimination index of 0.961 (95% confidence interval = 0.886-1.00). PFGE and ERIC-PCR showed a 90.4% correlation in the designation of isolates as clonal or non-clonal in pairwise comparisons. Both systems were highly reproducible and provided highly similar and supplementary data compared with serotyping regarding the transmission dynamics of shigellosis in this community. ERIC-PCR is considerably more rapid and inexpensive than PFGE and may have a complementary role to PFGE for initial investigations of hypothesized outbreaks in resource-limited settings.

Original languageEnglish (US)
Pages (from-to)1018-1025
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume86
Issue number6
DOIs
StatePublished - Jun 2012

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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