Facial subunit composite tissue allografts in nonhuman primates: I. technical and immunosuppressive requirements for prolonged graft Survival

Rolf N. Barth, Rachel Bluebond-Langner, Arthur Nam, Matthew Stanwix, Steven Shipley, Stephen T. Bartlett, Eduardo D. Rodriguez

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Widespread application of composite tissue allotransplantation has been impeded by risks of rejection and conventional immunosuppression. The authors have developed a nonhuman primate composite tissue allotransplantation model that demonstrated reliable and long-term success necessary to progress to preclinical studies. METHODS: Composite facial subunits (e.g., skin, muscle, and bone) were transplanted between mismatched cynomolgus monkeys. Vascular supply was based on the common carotid artery and external and internal jugular veins. Facial allografts were heterotopically transplanted to the recipient's lower abdomen with end-to-side anastomoses of the common carotid artery to the common femoral artery and of both the internal and external jugular veins to the common femoral vein. Animals received tacrolimus monotherapy. Grafts were inspected daily, submitted to biopsy regularly, and studied with magnetic resonance imaging. RESULTS: Thirteen transplants were performed. Two grafts based on the common carotid artery and only the internal jugular vein failed within 3 to 5 days because of venous thrombosis not related to rejection. Subsequent transplants included anastomoses of both the internal and external jugular veins to the common femoral vein without thromboses. Immunosuppression consisted of tacrolimus monotherapy and was tolerated without complications. Long-term success was achieved with rejection-free graft survival (60 to 177 days). CONCLUSIONS: The authors have developed the first successful model of facial composite tissue allotransplantation in a nonhuman primate. Technical requirements include preservation of both internal and external jugular venous outflow. Tacrolimus monotherapy permitted prolonged rejection-free graft survival without early complications. This model provides a platform for further investigation of composite tissue allotransplantation tolerance and requirements for indefinite survival.

Original languageEnglish (US)
Pages (from-to)493-501
Number of pages9
JournalPlastic and Reconstructive Surgery
Volume123
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

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Composite Tissue Allografts
Vascularized Composite Allotransplantation
Jugular Veins
Graft Survival
Immunosuppressive Agents
Primates
Common Carotid Artery
Tacrolimus
Transplants
Femoral Vein
Immunosuppression
Macaca fascicularis
Femoral Artery
Venous Thrombosis
Abdomen
Allografts
Blood Vessels
Thrombosis
Neck
Magnetic Resonance Imaging

ASJC Scopus subject areas

  • Surgery

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Facial subunit composite tissue allografts in nonhuman primates : I. technical and immunosuppressive requirements for prolonged graft Survival. / Barth, Rolf N.; Bluebond-Langner, Rachel; Nam, Arthur; Stanwix, Matthew; Shipley, Steven; Bartlett, Stephen T.; Rodriguez, Eduardo D.

In: Plastic and Reconstructive Surgery, Vol. 123, No. 2, 02.2009, p. 493-501.

Research output: Contribution to journalArticle

Barth, Rolf N. ; Bluebond-Langner, Rachel ; Nam, Arthur ; Stanwix, Matthew ; Shipley, Steven ; Bartlett, Stephen T. ; Rodriguez, Eduardo D. / Facial subunit composite tissue allografts in nonhuman primates : I. technical and immunosuppressive requirements for prolonged graft Survival. In: Plastic and Reconstructive Surgery. 2009 ; Vol. 123, No. 2. pp. 493-501.
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