Fabrication of poly(ethylene glycol): Gelatin methacrylate composite nanostructures with tunable stiffness and degradation for vascular tissue engineering

Peter Kim, Alex Yuan, Ki Hwan Nam, Alex Jiao, Deok Ho Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Although synthetic polymers are desirable in tissue engineering applications for the reproducibility and tunability of their properties, synthetic small diameter vascular grafts lack the capability to endothelialize in vivo. Thus, synthetically fabricated biodegradable tissue scaffolds that reproduce important aspects of the extracellular environment are required to meet the urgent need for improved vascular grafting materials. In this study, we have successfully fabricated well-defined nanopatterned cell culture substrates made of a biodegradable composite hydrogel consisting of poly(ethylene glycol) dimethacrylate (PEGDMA) and gelatin methacrylate (GelMA) by using UV-assisted capillary force lithography. The elasticity and degradation rate of the composite PEG-GelMA nanostructures were tuned by varying the ratios of PEGDMA and GelMA. Human umbilical vein endothelial cells (HUVECs) cultured on nanopatterned PEG-GelMA substrates exhibited enhanced cell attachment compared with those cultured on unpatterned PEG-GelMA substrates. Additionally, HUVECs cultured on nanopatterned PEG-GelM substrates displayed well-aligned, elongated morphology similar to that of native vascular endothelial cells and demonstrated rapid and directionally persistent migration. The ability to alter both substrate stiffness and degradation rate and culture endothelial cells with increased elongation and alignment is a promising next step in recapitulating the properties of native human vascular tissue for tissue engineering applications.

Original languageEnglish (US)
Article number024112
JournalBiofabrication
Volume6
Issue number2
DOIs
StatePublished - Jun 2014
Externally publishedYes

Keywords

  • biodegradable
  • gelatin methacrylate
  • hydrogel
  • poly(ethylene glycol)
  • vascular tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Fabrication of poly(ethylene glycol): Gelatin methacrylate composite nanostructures with tunable stiffness and degradation for vascular tissue engineering'. Together they form a unique fingerprint.

Cite this