Fabrication of electrospun silica-titania nanofibers with different silica content and evaluation of the morphology and osteoinductive properties

Xiaokun Wang, Jingxian Zhu, Ling Yin, Shize Liu, Xin Zhang, Yingfang Ao, Haifeng Chen

Research output: Contribution to journalArticle

Abstract

Ceramic-derived materials have shown enhanced osteogenic potential for bone tissue engineering applications. Silica is the major component of bioglass, and titania, the oxide complex of titanium, has been found to enhance osteoblast differentiation. In this study, three groups of sol-gelderived silica-titania fibrous meshes with precursor ratios of Ti:Si = 7:3, 1:1, 3:7 were fabricated by electrospinning. The effects of silica content on the crystal phase and morphology of silica-titania hybrid nanofiber meshes were also analyzed by scanning electron microscopy, X-ray diffraction, and laser confocal microscopy. The osteogenic potential of the silica-titania meshes was evaluated by seeding mesenchymal stem cells (MSCs) on each mesh and determining cell number, osteodifferentiation markers, and osteopontin production over time. Our results show that cells proliferated throughout the mesh surfaces with similar morphology in all groups. Decreased cell proliferation was observed with the fiber meshes compared with glass controls, whereas cell differentiation toward osteoblast was enhanced on the mesh groups, especially on the Ti:Si = 7:3 group. These findings suggest that higher fiber diameter, degree of crystallization, and titania content of nanofibers can enhance osteodifferentiation of MSCs.

Original languageEnglish (US)
Pages (from-to)3511-3517
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number12
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Nanofibers
Silicon Dioxide
Titanium
Silica
Fabrication
Osteoblasts
Stem cells
X ray lasers
Osteopontin
Fibers
Confocal microscopy
Bioelectric potentials
Cell proliferation
Electrospinning
Polymethyl Methacrylate
Bioactive glass
Crystallization
Tissue engineering
Sols
Bone

Keywords

  • Electrospinning
  • MSCs
  • Nanofibers
  • Osteoinductive
  • Silica-titania

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Fabrication of electrospun silica-titania nanofibers with different silica content and evaluation of the morphology and osteoinductive properties. / Wang, Xiaokun; Zhu, Jingxian; Yin, Ling; Liu, Shize; Zhang, Xin; Ao, Yingfang; Chen, Haifeng.

In: Journal of Biomedical Materials Research - Part A, Vol. 100 A, No. 12, 01.01.2012, p. 3511-3517.

Research output: Contribution to journalArticle

Wang, Xiaokun ; Zhu, Jingxian ; Yin, Ling ; Liu, Shize ; Zhang, Xin ; Ao, Yingfang ; Chen, Haifeng. / Fabrication of electrospun silica-titania nanofibers with different silica content and evaluation of the morphology and osteoinductive properties. In: Journal of Biomedical Materials Research - Part A. 2012 ; Vol. 100 A, No. 12. pp. 3511-3517.
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